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位置: 首页 > 标签抗体 > His-Tag Mouse Monoclonal Antibody (4E6)

His-Tag Mouse Monoclonal Antibody (4E6)#T523

His-Tag Mouse Monoclonal Antibody (4E6)
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产品总价:
产品详情

产品名称His-Tag Mouse Monoclonal Antibody (4E6)

来源种属Mouse

克隆性Monoclonal

克隆性4E6

纯化Affinity purification using immunogen.

应用WB

特异性The His tag antibody can recognize C-terminal, internal, and N-terminal His-tag fusion proteins..

基因/蛋白名称His-Tag

浓度1.0mg/ml

配方Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C

应用详情

Western blotting: 1:3000~1:10000

Western blot analysis of His recombinant protein, using #T523 diluted at 1£©1:10,000, 2£©1:20,000
背景

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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