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位置: 首页 > 磷酸化抗体 > Histone H3(Phospho-Thr32) Rabbit Polyclonal Antibody

Histone H3(Phospho-Thr32) Rabbit Polyclonal Antibody#12087

Histone H3(Phospho-Thr32) Rabbit Polyclonal Antibody
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产品总价:
产品详情

产品名称Histone H3(Phospho-Thr32) Rabbit Polyclonal Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Rt Ms

特异性The Histone H3(Phospho Thr32) Rabbit Polyclonal Antibody detects endogenous Histone H3 (Phospho Thr32) protein.

免疫原描述Peptide sequence around phosphorylation site of threonine 32(L-A-T(p)-K-A) derived from Human Histone H3.

基因/蛋白名称Histone H3(Phospho-Thr32)

修饰Phospho

别名H3 histone antibody; HIST1H3A antibody; Histone cluster 1; H3a antibody

数据库入口号Swiss-Prot#:P68431

UniprotP68431

实际分子量15-17kd

浓度1.0mg/ml

配方PBS, pH 7.4, containing 0.5%BSA,0.02% sodium azide as Preservative and 50% Glycerol.

保存Store at -20˚C

应用详情

Western blotting: 1:1000~2000

Western blot analysis of extracts from Hela cells, untreated (-) or treated with Nocodazole (1μg/ml, 24 hr; +), using #12087 diluted at 1:2,000.
背景

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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