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ITGA4 antibody#38151

ITGA4 antibody
ITGA4 antibody
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产品总价:
产品详情

产品名称ITGA4 antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were purified by affinity purification using immunogen.

应用WB,IHC

种属反应性Human,Mouse,Rat

特异性The antibody detects endogenous level of total ITGA4 protein.

免疫原类型Recombinant Protein

免疫原描述Recombinant protein of human ITGA4.

基因/蛋白名称ITGA4

别名ITGA4;CD49D;IA4;MGC90518;

数据库入口号Swiss-Prot#: P13612
NCBI Gene ID: 3676

UniprotP13612

实际分子量115kd

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C

应用详情
WB 1:500 - 1:2000
IHC 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using ITGA4 antibody at 1:1000 dilution.
Western blot analysis of extracts of various cell lines, using ITGA4 antibody at 1:1000 dilution.
背景
Integrins are α/β heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 α and 8 β subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with the extracellular environment (inside-out signaling) (1,2).A pair of important α4 integrins, α4β1 and α4β7, interact with VCAM-1, fibronectin, and MAdCAM-1 at cell adhesions (3). Gene knockout and antibody blocking research reveal that α4 integrins play important roles in embryonic liver and heart development and in fetal lymphocyte homing (4-6). Phosphorylation at Ser988 within the cytoplasmic tail of integrin α4 blocks binding to paxillin and promotes leading edge migration (7,8).On SDS-PAGE, integrin α4 can migrate at several different apparent molecular sizes, a 150 kDa mature protein and a 140 kDa precursor protein (a 180 kDa protein also exists under mild non-reducing conditions) (9). Integrin α4 has a cleavage site at Arg558, which results in a small portion of the protein as either an 80 kDa N-terminal or 70 kDa C-terminal fragment (10).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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