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位置: 首页 > 磷酸化抗体 > VEGFR2(Phospho-Tyr1214) Antibody

VEGFR2(Phospho-Tyr1214) Antibody#11085

VEGFR2(Phospho-Tyr1214) Antibody
VEGFR2(Phospho-Tyr1214) Antibody
VEGFR2(Phospho-Tyr1214) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称VEGFR2(Phospho-Tyr1214) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC IF

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of VEGFR2 only when phosphorylated at tyrosine 1214.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 1214 (F-H-Y(p)-D-N) derived from Human VEGFR2.

基因/蛋白名称VEGFR2

修饰Phospho

别名FLK1; KDR; VGFR2; VGR2; kinase insert domain receptor

数据库入口号Swiss-Prot: P35968
NCBI Protein: NP_002244.1

UniprotP35968

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 230kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from SKOV3 cells using VEGFR2(Phospho-Tyr1214) Antibody #11085(Lane 1) and the same antibody preincubated with blocking peptide(Lane2).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using VEGFR2(Phospho-Tyr1214) Antibody #11085(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed MCF cells using VEGFR2(Phospho-Tyr1214) Antibody #11085.
背景

Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions

Lamalice L, et al. (2004). Oncogene.23(2): 434-445.
Takahashi T, et al. (2001). EMBO J .20(11): 2768-2778.

如果您使用该产品11085发表了文章,请通知我们,让我们可以引用您的文献。

et al,Impaired tumor angiogenesis and VEGF-induced pathway in endothelial CD146 knockout mice.In Protein Cell on 2014 Jun by Qiqun Zeng , Zhenzhen Wu et al..PMID: 24756564, , (2014),
PMID: 24756564
et al,CD146 is a coreceptor for VEGFR-2 in tumor angiogenesis. In Blood on 2012 Sep 13 by Tianxia Jiang, Jie Zhuang, et al..PMID: 22718841, , (2012),
PMID: 22718841
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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