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ILF3 antibody#38409

ILF3 antibody
ILF3 antibody
ILF3 antibody
ILF3 antibody
ILF3 antibody
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产品总价:
产品详情

产品名称ILF3 antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were purified by affinity purification using immunogen.

应用WB,IHC,IF

种属反应性Human,Mouse,Rat

特异性The antibody detects endogenous level of total ILF3 protein.

免疫原类型Recombinant Protein

免疫原描述Recombinant protein of human ILF3.

基因/蛋白名称ILF3

别名CBTF; DRBF; MMP4; MPP4; NF90; NFAR; NF110; NF90a; NF90b; NFAR2; TCP80; DRBP76; NF110b; NFAR-1; TCP110; MPHOSPH4; NF-AT-90;

数据库入口号Swiss-Prot#: Q12906
NCBI Gene ID: 3609

UniprotQ12906

实际分子量95kd

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C

应用详情
Western blotting: 1:500 - 1:2000
Western blot analysis of extracts of various cell lines, using ILF3 antibody at 1:1000 dilution.
Western blot analysis of extracts of mouse thymus, using ILF3 antibody at 1:1000 dilution.
Immunofluorescence analysis of C6 cells using ILF3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using ILF3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using ILF3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
背景
This gene encodes a double-stranded RNA (dsRNA) binding protein that complexes with other proteins, dsRNAs, small noncoding RNAs, and mRNAs to regulate gene expression and stabilize mRNAs. This protein was first discovered to be a subunit of the nuclear factor of activated T-cells (NFAT); a transcription factor required for T-cell expression of interleukin 2. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the larger of which is the product of this gene. These proteins have been shown to affect the redistribution of nuclear mRNA to the cytoplasm. Knockdown of NF45 or NF90 protein retards cell growth; possibly by inhibition of mRNA stabilization. In contrast, an isoform (NF110) of this gene that is predominantly restricted to the nucleus has only minor effects on cell growth when its levels are reduced. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Nov 2008]

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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