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位置: 首页 > ELISA试剂盒 > Mouse Fibronectin ELISA Kit

Mouse Fibronectin ELISA Kit#EK5144

是否有货: 1-2周
产品总价:
产品详情

产品名称Mouse Fibronectin ELISA Kit

特异性Mouse

交叉反应性There is no detectable cross-reactivity with other relevant proteins.

免疫原类型from plasma

别名Integrin beta-1; Fibronectin receptor subunit beta; VLA-4 subunit beta; CD29; Itgb1;

数据库入口号P09055

细胞定位 Cell membrane; Colocalizes with ITGB1BP1 and metastatic suppressor proteinNME2 at the edge or peripheral ruffles and lamellipodia during theearly stages of cell spreading on fibronectin or collagen.

应用详情
sensitivity:15pg ml
Detect Range:156pg ml-10 000pg ml
sample_type:cell culture supernates serum and plasma(heparin EDTA citrate).
capture_antibody:monoclonal antibody from rat
detection_antibody:polyclonal antibody from goat
gene_name:FN1
protein_name:Fibronectin
gene_full_name:Integrin beta-1
tissue_specificity: Isoform 2 is expressed in skeletal and cardiacmuscles only (at protein level). Isoform 1 is very weaklyexpressed in striated muscles and not detected in adult skeletalmuscle fibers and cardiomyocytes..
sequence_similarities:Belongs to the integrin beta chain family.
tmb_incubation:20-25min
research_category:FN1
产品描述
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Fibronectin
背景
protein_function: Integrins alpha-1,beta-1, alpha-2,beta-1, alpha-10,beta-1 and alpha-11,beta-1 are receptors for collagen. Integrins alpha-1,beta-1 and alpha-2,beta-2 recognize the proline-hydroxylatedsequence G-F-P-G-E-R in collagen. Integrins alpha-2,beta-1, alpha-3,beta-1, alpha-4,beta-1, alpha-5,beta-1, alpha-8,beta-1, alpha-10,beta-1, alpha-11,beta-1 and alpha-V,beta-1 are receptors forfibronectin. Alpha-4,beta-1 recognizes one or more domains withinthe alternatively spliced CS-1 and CS-5 regions of fibronectin.Integrin alpha-5,beta-1 is a receptor for fibrinogen. Integrinalpha-1,beta-1, alpha-2,beta-1, alpha-6,beta-1 and alpha-7,beta-1are receptors for lamimin. Integrin alpha-4,beta-1 is a receptorfor VCAM1 and recognizes the sequence Q-I-D-S in VCAM1. Integrinalpha-9,beta-1 is a receptor for VCAM1, cytotactin andosteopontin. It recognizes the sequence A-E-I-D-G-I-E-L incytotactin. Integrin alpha-3,beta-1 is a receptor for epiligrin,thrombospondin and CSPG4. Integrin alpha-3,beta-1 provides adocking site for FAP (seprase) at invadopodia plasma membranes ina collagen-dependent manner and hence may participate in theadhesion, formation of invadopodia and matrix degradationprocesses, promoting cell invasion. Alpha-3,beta-1 may mediatewith LGALS3 the stimulation by CSPG4 of endothelial cellsmigration. Integrin alpha-V,beta-1 is a receptor for vitronectin.Beta-1 integrins recognize the sequence R-G-D in a wide array ofligands. When associated with alpha-7,beta-1 integrin, regulatescell adhesion and laminin matrix deposition. Involved in promotingendothelial cell motility and angiogenesis. Involved in osteoblastcompaction through the fibronectin fibrillogenesis cell-mediatedmatrix assembly process and the formation of mineralized bonenodules. May be involved in up-regulation of the activity ofkinases such as PKC via binding to KRT1. Together with KRT1 andGNB2L1, serves as a platform for SRC activation or inactivation.Plays a mechanistic adhesive role during telophase, required forthe successful completion of cytokinesis..
Fibronectin(FN) also known as LETS, is identified on the surfFN of fibroblasts by labeling with radioactive compounds or specific antibodies. Fibronectin is a 430,000-dalton dimeric glycoprotein that exists in 2 forms, termed cellular and plasma fibronectin. Cellular and plasma fibronectins are heterodimers consisting of similar but not identical polypeptides. These two forms of FN differ in biologic activity. Fibronectins bind cell surfFNs and various compounds including collagen, fibrin, heparin, DNA, and actin. Because fibronectin stimulates endocytosis in several systems and promotes the clearance of particulate material from the circulation, it could function in the clearance of C1q-coated material such as immune complexes or cellular debris. Fibronectins are involved in cell adhesion, cell motility, opsonization, would healing, and maintenance of cell shape. LETS, encoded on chromosome 8, is responsible for the LETS protein expression in humans. Because LETS has been implicated in tumorigenicity and cellular transformation, it is of interest that rearrangement or modifications in the number of chromosome 8 have been associated with certain forms of cancer.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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