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GRP78 BiP Antibody#48119

GRP78 BiP Antibody
GRP78 BiP Antibody
GRP78 BiP Antibody
是否有货: Yes
产品总价:
产品详情

产品名称GRP78 BiP Antibody

来源种属Mouse

克隆性Monoclonal

克隆性C9-9

纯化ProA affinity purified

应用WB, ICC, IHC

种属反应性Hu, Ms

免疫原描述recombinant protein

别名78 kDa glucose regulated protein antibody
78 kDa glucose-regulated protein antibody
AL022860 antibody
AU019543 antibody
BIP antibody
D2Wsu141e antibody
D2Wsu17e antibody
Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody
Endoplasmic reticulum lumenal Ca2+ binding protein grp78 antibody
Epididymis secretory sperm binding protein Li 89n antibody
FLJ26106 antibody
Glucose Regulated Protein 78kDa antibody
GRP 78 antibody
GRP-78 antibody
GRP78 antibody
GRP78_HUMAN antibody
Heat shock 70 kDa protein 5 antibody
Heat Shock 70kDa Protein 5 antibody
Heat shock protein family A (Hsp70) member 5 antibody
HEL S 89n antibody
Hsce70 antibody
HSPA 5 antibody
HSPA5 antibody
Immunoglobulin Heavy Chain Binding Protein antibody
Immunoglobulin heavy chain-binding protein antibody
mBiP antibody
MIF2 antibody
Sez7 antibody

数据库入口号Swiss-Prot#:P11021

计算分子量78kDa

配方1*TBS (pH7.4), 0.5%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

保存Store at -20˚C

应用详情
WB: 1:2,000-1:5,000

ICC: 1:50-1:100

Western blot analysis on cell lysates using anti- GRP78 mouse mAb.
ICC staining GRP78 in MCF-7 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining GRP78 in HepG2 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
背景
Binding immunoglobulin protein (BiP) also known as 78 kDa glucose-regulated protein (GRP-78) or heat shock 70 kDa protein 5 (HSPA5) is a protein that in humans is encoded by the HSPA5 gene. BiP is a HSP70 molecular chaperone located in the lumen of the endoplasmic reticulum (ER) that binds newly synthesized proteins as they are translocated into the ER, and maintains them in a state competent for subsequent folding and oligomerization. BiP is also an essential component of the translocation machinery, as well as playing a role in retrograde transport across the ER membrane of aberrant proteins destined for degradation by the proteasome. Like many stress and heat shock proteins, BiP/GRP78 has potent immunological activity when released from the internal environment of the cell into the extracelluar space.specifically, it feeds anti-inflammatory and pro-resolutory signals into immune networks, thus helping to resolve inflammation.
背景文献
1."Human XTP3-B forms an endoplasmic reticulum quality control scaffold with the HRD1-SEL1L ubiquitin ligase complex and BiP." Hosokawa N., Wada I., Nagasawa K., Moriyama T., Okawa K., Nagata K. J. Biol. Chem. 283:20914-20924(2008)
2."Crystal structures of the ATPase domains of four human Hsp70 isoforms: HSPA1L/Hsp70-hom, HSPA2/Hsp70-2, HSPA6/Hsp70B', and HSPA5/BiP/GRP78." Wisniewska M., Karlberg T., Lehtio L., Johansson I., Kotenyova T., Moche M., Schuler H. PLoS ONE 5:E8625-E8625(2010)
3."Adenosine-derived inhibitors of 78 kDa glucose regulated protein (Grp78) ATPase: insights into isoform selectivity." Macias A.T., Williamson D.S., Allen N., Borgognoni J., Clay A., Daniels Z., Dokurno P., Drysdale M.J., Francis G.L., Graham C.J., Howes R., Matassova N., Murray J.B., Parsons R., Shaw T., Surgenor A.E., Terry L., Wang Y., Wood M., Massey A.J. J. Med. Chem. 54:4034-4041(2011)

如果您使用该产品48119发表了文章,请通知我们,让我们可以引用您的文献。

et al,Sestrin2 Overexpression Ameliorates Endoplasmic Reticulum Stress-Induced Apoptosis via Inhibiting mTOR Pathway in HepG2 Cells. In Int J Endocrinol on 2022 Dec 10 by Huiling Hu, Zhijun Luo, et al..PMID:36536875, , (2022),
PMID: 36536875
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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