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位置: 首页 > 抗体 > NF-κB p65 Antibody

NF-κB p65 Antibody#48498

NF-κB p65 Antibody
NF-κB p65 Antibody
NF-κB p65 Antibody
NF-κB p65 Antibody
是否有货: Yes
产品总价:
产品详情

产品名称NF-κB p65 Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Peptide affinity purified

应用WB, IHC, FC

种属反应性Hu, Ms, Rt

免疫原描述This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to N-terminal NF-κB p65.

别名Avian reticuloendotheliosis viral (v rel) oncogene homolog A antibody
MGC131774 antibody
NF kappa B p65delta3 antibody
NFKB3 antibody
Nuclear Factor NF Kappa B p65 Subunit antibody
Nuclear factor NF-kappa-B p65 subunit antibody
Nuclear factor of kappa light polypeptide gene enhancer in B cells 3 antibody
Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 antibody
OTTHUMP00000233473 antibody
OTTHUMP00000233474 antibody
OTTHUMP00000233475 antibody
OTTHUMP00000233476 antibody
OTTHUMP00000233900 antibody
p65 antibody
p65 NF kappaB antibody
p65 NFkB antibody
relA antibody
TF65_HUMAN antibody
Transcription factor p65 antibody
v rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B cells 3 (p65)) antibody
V rel avian reticuloendotheliosis viral oncogene homolog A antibody
v rel reticuloendotheliosis viral oncogene homolog A (avian) antibody
V rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B cells 3, p65 antibody

数据库入口号Swiss-Prot#:Q04206

计算分子量65 kDa

配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

保存Store at -20˚C

应用详情
WB: 1:1,000-1:2,000
IHC: 1:100-1:200


FC: 1:50-1:100
Western blot analysis of NF-κB p65 on different lysates using anti-NF-κB p65 antibody at 1/1000 dilution.

Positive control:

Lane1: Hela

Lane2:A549

Lane3: PC12

Lane 4: Mouse embryonic stem cell

Lane5: NIH/3T3



Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-NF-κB p65 antibody. Counter stained with hematoxylin.

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-NF-��B p65 antibody. Counter stained with hematoxylin.

Flow cytometric analysis of Hela cells with NF-κB p65 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.

背景
NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins. NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression.
背景文献
1."Duration of nuclear NF-kappaB action regulated by reversible acetylation." Chen L.F., Fischle W., Verdin E., Greene W.C.Science 293:1653-1657(2001)
2."A novel protein overexpressed in hepatoma accelerates export of NF-kappa B from the nucleus and inhibits p53-dependent apoptosis." Higashitsuji H., Higashitsuji H., Nagao T., Nonoguchi K., Fujii S., Itoh K., Fujita J. Cancer Cell 2:335-346(2002)
3."Breast cancer metastasis suppressor 1 functions as a corepressor by enhancing histone deacetylase 1-mediated deacetylation of RelA/p65 and promoting apoptosis." Liu Y., Smith P.W., Jones D.R. Mol. Cell. Biol. 26:8683-8696(2006)
4."SIRT2 regulates NF-kappaB dependent gene expression through deacetylation of p65 Lys310." Rothgiesser K.M., Erener S., Waibel S., Luscher B., Hottiger M.O. J. Cell Sci. 123:4251-4258(2010)

如果您使用该产品48498发表了文章,请通知我们,让我们可以引用您的文献。

et al,A multi-omics approach based on 1H-NMR metabonomics combined with target protein analysis to reveal the mechanism of RIAISs on cervical carcinomaIn Aging (Albany NY)On2022 Sep 27byChai Yanlan , Aibibai Jielili et al..PMID:36170024, , (2023),
PMID: 36170024
et al,Effect of Fushengong Decoction on PTEN/PI3K/AKT/NF-KB Pathway in Rats With Chronic Renal Failure via Dual-Dimension Network Pharmacology Strategy.In Front Pharmacol on 2022 Mar 15 by Hongyu Luo, Munan Wang,et al..PMID: 35370667, , (2022),
PMID: 35370667
et al,Resveratrol attenuates cigarette smoke extract induced cellular senescence in human airway epithelial cells by regulating the miR-34a/SIRT1/NF-κB pathway. In Medicine (Baltimore) on 2022 Nov 18 by Xiao-Li Zeng, Xin-Na Yang, et al..PMID: 36401446, , (2022),
PMID: 36401446
et al,Effect of Fushengong Decoction on PTEN/PI3K/AKT/NF-κB Pathway in Rats With Chronic Renal Failure via Dual-Dimension Network Pharmacology Strategy. In Front Pharmacol on 2022 Mar 15 by Hongyu Luo, Munan Wang, et al..PMID: 35370667, , (2022),
PMID: 35370667
et al,Salinomycin promotes T-cell proliferation by inhibiting the expression and enzymatic activity of immunosuppressive indoleamine-2,3-dioxygenase in human breast cancer cells. In Toxicol Appl Pharmacol on 2020 Oct 1 by Yuwen Sheng, Zhonghui Zhang, et al..PMID:32822738, , (2020),
PMID: 32822738
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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