产品详情
产品名称LRP1 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆性SA0290
纯化ProA affinity purified
应用WB, ICC/IF, IHC, IP, FC
种属反应性Hu, Ms, Rt
免疫原描述recombinant protein
别名A2MR antibody Alpha 2 macroglobulin receptor antibody alpha 2MR antibody Alpha-2-macroglobulin receptor antibody APOER antibody Apolipoprotein E receptor antibody APR antibody CD 91 antibody CD91 antibody CD91 antigen antibody IGFBP3R antibody LDL receptor related protein 1 antibody Low density lipoprotein receptor related protein 1 antibody Low density lipoprotein related protein 1 antibody Low-density lipoprotein receptor-related protein 1 intracellular domain antibody LRP 1 antibody LRP 515 antibody LRP 85 antibody LRP antibody LRP ICD antibody LRP-1 antibody LRP-515 antibody LRP-85 antibody Lrp1 antibody LRP1 protein antibody LRP1_HUMAN antibody LRP1A antibody LRP515 antibody LRP85 antibody LRPICD antibody MGC88725 antibody Prolow density lipoprotein receptor related protein 1 antibody TbetaR V/LRP 1/IGFBP 3 receptor antibody TbetaRV/LRP1/IGFBP3 receptor antibody TGFBR 5 antibody TGFBR5 antibody Type V tgf beta receptor antibody
数据库入口号Swiss-Prot#:Q07954
计算分子量85 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC: 1:10-1:100
Western blot analysis of LRP1 on different lysates using anti-LRP1 antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse liver Lane 2: Mouse brain Lane Lane 3: Mouse lung Lane 4: Human liver Lane 5: HepG2 Lane 6: Human lung
Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-LRP1 antibody. Counter stained with hematoxylin.
ICC staining LRP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS..
ICC staining LRP1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining LRP1 in HUVEC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with LRP1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
背景
Members of the LDL receptor gene family, including LDLR (low density lipoprotein receptor), LRP1 (low density lipoprotein related protein), Megalin (also designated GP330), VLDLR (very low density lipoprotein receptor) and ApoER2 are characterized by a cluster of cysteine-rich class A repeats, epidermal growth factor (EGF)-like repeats, YWTD repeats and an O-linked sugar domain. LRP1, also designated LRP and ?-2-Macroglobulin receptor, is an endocytic receptor that mediates the uptake of at least 15 ligands, including ?-2-Macroglobulin and apoE. LRP1 is cleaved into a membrane subunit and an extracellular subunit, which remain non-covalently associated. Proper folding and trafficking of LRP1 is facilitated by the receptor-associated protein (RAP), a molecular chaperone. The uptake of all known ligands through LRP1 can be blocked by RAP, which induces a conformational change in the receptor that renders it unable to bind ligands. LRP1, which is expressed in brain, liver and lung, is also implicated in Alzheimer��s disease (AD), as the human LRP gene localizes to a potential AD locus on chromosome 12.
背景文献
1. Fernandez-Castaneda, A. et al. 2013. Identification of the low density lipoprotein (LDL) receptor-related protein-1 interactome in central nervous system myelin suggests a role in the clearance of necrotic cell debris. J. Biol. Chem. 288: 4538-4548. 2. Yahiro, K. et al. 2012. Low-density lipoprotein receptor-related protein-1 (LRP1) mediates autophagy and apoptosis caused by Helicobacter pylori VacA. J. Biol. Chem. 287: 31104-31115.
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et al,Lactotransferrin Downregulation Drives the Metastatic Progression in Clear Cell Renal Cell Carcinoma. In Cancers (Basel) on 2020 Mar 31 by I-Jen Chiu, Yung-Ho Hsu, et al..PMID: 32244557,
, (2020),
PMID: 32244557
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