免费电话:400-9961-933
邮箱:techcn@signalwayantibody.com
欢迎光临本站
登录 注册 English
位置: 首页 > 重组兔单抗 > CD86 Rabbit mAb

CD86 Rabbit mAb#48763

评价
Recombinant
CD86 Rabbit mAb
CD86 Rabbit mAb
CD86 Rabbit mAb
CD86 Rabbit mAb
CD86 Rabbit mAb
CD86 Rabbit mAb
是否有货: Yes
产品总价:
产品详情

产品名称CD86 Rabbit mAb

克隆性SJ20-00

纯化ProA affinity purified

应用WB, ICC/IF, IHC, IP, FC

种属反应性Hu Ms

免疫原描述Synthetic peptide within Human CD86 aa 1-50 / 329.

别名Activation B7-2 antigen 3 antibody
Activation B7-2 antigen antibody
B-lymphocyte activation antigen B7-2 2 antibody
B-lymphocyte activation antigen B7-2 antibody
B7 2 antibody
B7 antibody
B7-2 antibody
B7.2 antibody
B70 antibody
B72 antigen antibody
BU63 antibody
CD28 antigen ligand 2 2 antibody
CD28 antigen ligand 2 antibody
Cd28l2 antibody
CD28LG2 antibody
CD86 antibody
CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) 1, 2 antibody
CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) antibody
CD86 antigen antibody
CD86 molecule antibody
CD86_HUMAN antibody
CLS1 antibody
CTLA-4 counter-receptor B7.2 2, 3 antibody
CTLA-4 counter-receptor B7.2 antibody
Early T-cell costimulatory molecule 1 antibody
ETC-1 antibody
FUN 1 antibody
FUN-1 antibody
LAB72 antibody
Ly-58 antibody
Ly58 antibody
MB7 antibody
MB7-2 antibody
Membrane glycoprotein antibody
MGC34413 antibody
T-lymphocyte activation antigen CD86 antibody
TS/A-2 antibody

数据库入口号Swiss-Prot#:P42081

计算分子量38 kDa

配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

保存Store at -20˚C

应用详情

WB: 1:500-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC: 1:50-1:100
IP: Use at an assay dependent concentration.

Western blot analysis of CD86 on different lysates using anti-CD86 antibody at 1/1,000 dilution. Positive control:
Lane 1: Raji
Lane 2: Jurkat
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD86 antibody. Counter stained with hematoxylin.
ICC staining CD86 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining CD86 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining CD86 in JAR cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of K562 cells with CD86 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
背景

T cell proliferation and lymphokine production are triggered by occupation of the TCR by antigen, followed by a costimulatory signal that is delivered by a ligand expressed on antigen presenting cells. The B7-related cell surface proteins B7-1 (CD80) and B7-2 (CD86) expressed on antigen presenting cells bind the homologous T cell receptors CD28 and CTLA-4 (cytotoxic T lymphocyte-associated protein-4) and trigger costimulatory signals for optimal T cell activation. CTLA-4 shares 31% overall amino acid identity with CD28, and it has been proposed that CD28 and CTLA-4 are functionally redundant. SLAM is a novel receptor on T cells that, when engaged, potentiates T cell expansion in a CD28-independent manner. B7, also designated BB1, is another ligand or counterreceptor for CD28 and CTLA-4 that is expressed on the antigen-presenting cell.

背景文献

1. Li Y & Ding J Optimized generation of survivin-specific cytotoxic T lymphocytes against lung cancer. Mol Med Rep 12:2169-74 (2015).
2. Tarhini AA et al. Immune monitoring of the circulation and the tumor microenvironment in patients with regionally advanced melanoma receiving neoadjuvant ipilimumab. PLoS One 9:e87705 (2014).

如果您使用该产品48763发表了文章,请通知我们,让我们可以引用您的文献。

et al,Preliminary Study on the Antigen-Removal from Extracellular Matrix via Different Decellularization. In Tissue Eng Part C Methods on 2022 Jun by Huan Wu, Guangfu Yin, et al..PMID: 35596569, , (2022),
PMID: 35596569
et al,Human Umbilical Cord Mesenchymal Stem Cells Improve the Necrosis and Osteocyte Apoptosis in Glucocorticoid-Induced Osteonecrosis of the Femoral Head Model through Reducing the Macrophage Polarization. In Int J Stem Cells on 2022 May 30 by Gang Tian, Chuanjie Liu, et al..PMID:34965999, , (2022),
PMID: 34965999
et al,Human Umbilical Cord Mesenchymal Stem Cells Improve the Necrosis and Osteocyte Apoptosis in Glucocorticoid-Induced Osteonecrosis of the Femoral Head Model through Reducing the Macrophage Polarization. In Int J Stem Cells on 2021 Dec 31 by Gang Tian, Chuanjie Liu,al..PMID:34965999, , (2021),
PMID: 34965999
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

联系我们

Location :

南京市高新开发区星火路10号人才大厦C座

Phone :

400-9961-933
025-58868422

Email Address

techcn@signalwayantibody.com