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位置: 首页 > 重组兔单抗 > Phospho-Akt1(Ser473) Rabbit mAb

Phospho-Akt1(Ser473) Rabbit mAb#13357

评价
Recombinant
Phospho-Akt1(Ser473) Rabbit mAb
Phospho-Akt1(Ser473) Rabbit mAb
Phospho-Akt1(Ser473) Rabbit mAb
是否有货: Yes
产品总价:
产品详情

产品名称Phospho-Akt1(Ser473) Rabbit mAb

来源种属Rabbit

克隆性Monoclonal

克隆性SY28-05

纯化ProA affinity purified

应用WB, ICC/IF, IHC

种属反应性Hu, Ms

免疫原描述Synthetic phospho-peptide corresponding to residues surrounding Ser473 of human Akt1.

别名AKT 1 antibody
AKT antibody
AKT1 antibody
AKT1_HUMAN antibody
MGC99656 antibody
PKB antibody
PKB-ALPHA antibody
PRKBA antibody
Protein Kinase B Alpha antibody
Protein kinase B antibody
Proto-oncogene c-Akt antibody
RAC Alpha antibody
RAC antibody
RAC-alpha serine/threonine-protein kinase antibody
RAC-PK-alpha antibody

数据库入口号Swiss-Prot#:P31749

计算分子量56 kDa

配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

保存Store at -20˚C

应用详情

WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200

Western blot analysis of Phospho-Akt1(Ser473) on different lysates using anti-Phospho-Akt1(Ser473) antibody at 1/1,000 dilution. Positive control:
Lane 1: NIH/3T3 treated with PDGF
Lane 2: NIH/3T3 untreated
Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Phospho-Akt1(Ser473) antibody. Counter stained with hematoxylin.
ICC staining Phospho-Akt1(Ser473) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
背景

The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKBβ or RacPK-β) and Akt 3 (also designated PKBγ or thyoma viral proto-oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-β tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1(IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Phosphorylation of both residues is important to generate a high level of Akt1 activity, and the phosphorylation of Thr 308 is not dependent on phosphorylation of Ser 473 in vivo. Thus, Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s). The activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin, suggesting that the protein signals downstream of the PI kinases.

背景文献

1. Chen J et al. Low expression of phosphatase and tensin homolog in clear-cell renal cell carcinoma contributes to chemoresistance through activating the Akt/HDM2 signaling pathway. Mol Med Rep 12:2622-8 (2015).
2. Burdine LJ et al. Proteomic Identification of DNA-PK Involvement within the RET Signaling Pathway. PLoS One 10:e0127943 (2015).

如果您使用该产品13357发表了文章,请通知我们,让我们可以引用您的文献。

et al,Downregulation of GLYAT Facilitates Tumor Growth and Metastasis and Poor Clinical Outcomes Through the PI3K/AKT/Snail Pathway in Human Breast Cancer. In Front Oncol on 2021 Apr 22 by Xin Tian, Lina Wu,et al..PMID:33968740, , (2021),
PMID: 33968740
et al,7?dehydrocholesterol suppresses melanoma cell proliferation and invasion via Akt1/NF?kB signaling,In Oncol Lett on 2020 Dec by Jia Liu, Feiliang Zhong, et al..PMID: 33193858, , (2020),
PMID: 33193858
et al,NVD-BM-mediated genetic biosensor triggers accumulation of 7-dehydrocholesterol and inhibits melanoma via Akt1/NF-?B signaling. In Aging (Albany NY) on 2020 Jul 25 by Jia Liu, Lei Cao, et al..PMID: 32712598, , (2020),
PMID: 32712598
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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