产品详情
产品名称Caspase-1 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆性SU40-07
纯化ProA affinity purified
应用WB, IHC, FC
种属反应性Hu, Ms, Rt
免疫原描述recombinant protein
别名CASP-1 antibody
CASP1 antibody
CASP1_HUMAN antibody
Caspase 1 antibody
Caspase-1 subunit p10 antibody
ICE antibody
IL-1 beta-converting enzyme antibody
IL-1BC antibody
IL1 beta converting enzyme antibody
IL1B convertase antibody
Interleukin 1 beta convertase antibody
Interleukin 1B converting enzyme antibody
Interleukin-1 beta convertase antibody
Interleukin-1 beta-converting enzyme antibody
p45 antibody
数据库入口号Swiss-Prot#:P29466
计算分子量45 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
FC: 1:50-1:100
Western blot analysis of Caspase-1 on different lysates using anti-Caspase-1 antibody at 1/1,000 dilution. Positive control: Lane 1: THP-1 Lane 2: Jurkat
Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Caspase-1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Caspase-1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Caspase-1 antibody. Counter stained with hematoxylin.
Flow cytometric analysis of Hela cells with Caspase-1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
背景
Caspase-1, originally designated ICE (for IL-1 converting enzyme), is a member of the group of caspases with large prodomains. Caspase-1 promotes maturation of interleukin IL-1β and interleukin18 (IL-18) by proteolytic cleavage of precursor forms into biologically active pro-inflamatory cytokines. Active caspase-1, a (p20/p10)2 tetramer, is necessary and sufficient for cleavage of precursor IL-1 as well as for induction of apoptosis in some cell lines. The highly conserved family of caspases mediate many of the morphological and biochemical features of apoptosis, including structural dismantling of cell bodies and nuclei, fragmentation of genomic DNA, destruction of regulatory proteins and propagation of other pro-apoptotic molecules. The human Caspase-1 gene maps to chromosome 2q14 and encodes a cytoplasmic protein expressed in liver, heart, skeletal muscle kidney and testis. Caspase-1 has been implicated in inflammation, septic shock, and other situations such as wound healing and the growth of certain leukemias.
背景文献
1. Zhao J et al. Synthetic Oligodeoxynucleotides Containing Multiple Telemeric TTAGGG Motifs Suppress Inflammasome Activity in Macrophages Subjected to Oxygen and Glucose Deprivation and Reduce Ischemic Brain Injury in Stroke-Prone Spontaneously Hypertensive Rats. PLoS One 10:e0140772 (2015).
2. Zhang X et al. Porcine Mx1 fused to HIV Tat protein transduction domain (PTD) inhibits classical swine fever virus infection in vitro and in vivo. BMC Vet Res 11:264 (2015).
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