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位置: 首页 > 重组兔单抗 > NQO1 Rabbit mAb

NQO1 Rabbit mAb#49364

评价
Recombinant
NQO1 Rabbit mAb
NQO1 Rabbit mAb
是否有货: Yes
产品总价:
产品详情

产品名称NQO1 Rabbit mAb

来源种属Recombinant Rabbit

克隆性 Monoclonal antibody

克隆性JF440-1

纯化ProA affinity purified

应用WB, ICC/IF, IP, FC

种属反应性Hu, Ms, Rt

免疫原描述recombinant protein

别名Azoreductase antibody
Cytochrome b 5 reductase antibody
DHQU antibody
DIA 4 antibody
DIA4 antibody
Diaphorase (NADH/NADPH) (cytochrome b 5 reductase) antibody
Diaphorase (NADH/NADPH) antibody
Diaphorase 4 antibody
Dioxin inducible 1 antibody
DT diaphorase antibody
DT-diaphorase antibody
DTD antibody
Menadione reductase antibody
NAD(P)H dehydrogenase [quinone] 1 antibody
NAD(P)H dehydrogenase quinone 1 antibody
NAD(P)H menadione oxidoreductase 1 dioxin inducible antibody
NAD(P)H: menadione oxidoreductase 1 dioxin inducible 1 antibody
NAD(P)H:menadione oxidoreductase 1 antibody
NAD(P)H:Quinone acceptor oxidoreductase type 1 antibody
NAD(P)H:quinone oxidoreductase 1 antibody
NAD(P)H:quinone oxireductase antibody
NMOR 1 antibody
NMOR I antibody
NMOR1 antibody
NMORI antibody
NQO 1 antibody
NQO1 antibody
NQO1_HUMAN antibody
Phylloquinone reductase antibody
QR 1 antibody
QR1 antibody
Quinone reductase 1 antibody

数据库入口号Swiss-Prot#:P15559

计算分子量31/27 kDa

配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

保存Store at -20˚C

应用详情
WB: 1:1,000-5,000

ICC: 1:50-1:200
IP: 1:50
FC: 1:50-1:100
Western blot analysis of NQO1 on different lysates using anti-NQO1 antibody at 1/1,000 dilution. Positive control:
Lane 1: SH-SY-5Y
Lane 2: Mouse kidney
Lane 3: Raji
Flow cytometric analysis of Hela cells with NQO1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
背景
NAD(P)H:quinone oxidoreductase 1 (NQO1) and NRH:quinone oxidoreductase (NQO2) are flavoproteins that catalyze the metabolic detoxification of quinones and their derivatives to hydroquinones, using either NADH or NADPH as the electron donor. This protects cells against quinone-induced oxidative stress, cytotoxicity, and mutagenicity. Many tumors overexpress NQO1, which is an obligate two-electron reductase that deactivates toxins and activates bioreductive anticancer drugs. NQO1, a 274 amino acid protein, is ubiquitously expressed, but the expression level varies among tissues. NQO1 gene expression is coordinately induced in response to xenobiotics, antioxidants, heavy metals and radiation. The antioxidant response element (ARE) in the NQO1 gene promoter is essential for expression and coordinated induction of NQO1. ARE activation by tert-butylhydroquinone is dependent on PI3-kinase, which lies upstream of Nrf2. Nrf2, c-Jun, Nrf1, Jun-B and Jun-D bind to the ARE and regulate expression and induction of NQO1 gene. Maf-Maf homodimers and possibly Maf-Nrf2 heterodimers play a role in negative regulation of ARE-mediated transcription, but Maf-Nrf1 heterodimers fail to bind with the NQO1 gene ARE and do not repress NQO1 transcription.
背景文献
1. Greco T et al. Ketogenic diet decreases oxidative stress and improves mitochondrial respiratory complex activity. J Cereb Blood Flow Metab 36:1603-13 (2016).
2. Kigoshi Y et al. CACUL1/CAC1 Regulates the Antioxidant Response by Stabilizing Nrf2. Sci Rep 5:12857 (2015).

如果您使用该产品49364发表了文章,请通知我们,让我们可以引用您的文献。

et al,Asperuloside suppressing oxidative stress and inflammation in DSS-induced chronic colitis and RAW 264.7 macrophages via Nrf2/HO-1 and NF-kB pathways. In Chem Biol Interact on 2021 Aug 1 by Yong-Er Chen , Shi-Jie Xu et al..PMID:33974900, , (2021), , , (2021), , (2021),
PMID: 33974900
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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