产品详情
产品名称XBP1 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆性JM10-62
纯化ProA affinity purified
应用WB,IHC,ICC/IF, FC
种属反应性Hu
免疫原描述recombinant protein
别名Tax responsive element binding protein 5 antibody
Tax-responsive element-binding protein 5 antibody
TREB5 antibody
X box binding protein 1 antibody
X box binding protein 2 antibody
X-box-binding protein 1 antibody
XBP 1 antibody
XBP-1 antibody
XBP1 antibody
XBP1_HUMAN antibody
XBP2 antibody
数据库入口号Swiss-Prot#:P17861
计算分子量40 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:1,000
IHC: 1:50-1:200
ICC: 1:100-1:500
FC: 1:50-1:100
Western blot analysis of XBP1 on different cell lysates using anti-XBP1 antibody at 1/1,000 dilution. Positive control: Lane 1:HepG2 Lane 2: 293T
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-XBP1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-XBP1 antibody. Counter stained with hematoxylin.
ICC staining XBP1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining XBP1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining XBP1 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of 293T cells with XBP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
背景
The X-box binding protein-1 (XBP-1 or hXBP-1), also designated tax-responsive element-binding protein 5 (TREB5) in mouse and human, or hepatocarcinogenesis-related transcription factor (HTF) in rat, belongs to the basic region/leucine zipper (bZIP) family of transcription factors. XBP-1 was first characterized as a protein that binds to the HLA-DRα promoter in B cells. XBP-1 recognizes the cAMP responsive element (CRE) in enhancers of human T cell leukemia virus and major histocompatibility complex class II genes and activates transcription of these genes. It is expressed at high levels in developing bone and its levels are modulated during osteoblast development, suggesting a role in regulation of expression of osteoblast-specific genes. In addition to binding to CRE sequences, XBP-1 has been shown to bind to TPA response elements (TREs).
背景文献
1. Krawczyk KK et al. Assessing the contribution of thrombospondin-4 induction and ATF6a activation to endoplasmic reticulum expansion and phenotypic modulation in bladder outlet obstruction. Sci Rep 6:32449 (2016).
2. Prell T et al. The unfolded protein response in models of human mutant G93A amyotrophic lateral sclerosis. Eur J Neurosci 35:652-60 (2012).
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et al,X-box binding protein 1 (XBP1): a potential role in chemotherapy response, clinical pathologic features, non-inflamed tumour microenvironment for breast cancer. In Biosci Rep on 2022 Jun 30 by Zhipeng Zhu, Hongliang Zhan, et al..PMID:35543228,
, (2022),
PMID: 35543228
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