产品详情
产品名称MMP9 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆性JA80-73
纯化ProA affinity purified
应用WB, ICC/IF, IHC, FC
种属反应性Hu, Ms, Rt
免疫原描述recombinant protein
别名82 kDa matrix metalloproteinase-9 antibody
92 kDa gelatinase antibody
92 kDa type IV collagenase antibody
CLG 4B antibody
CLG4B antibody
Collagenase Type 4 beta antibody
Collagenase type IV 92 KD antibody
EC 3.4.24.35 antibody
Gelatinase 92 KD antibody
Gelatinase B antibody
Gelatinase beta antibody
GelatinaseB antibody
GELB antibody
Macrophage gelatinase antibody
MANDP2 antibody
Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) antibody
Matrix Metalloproteinase 9 antibody
MMP 9 antibody
MMP-9 antibody
MMP9 antibody
MMP9_HUMAN antibody
Type V collagenase antibody
数据库入口号Swiss-Prot#:P14780
计算分子量100 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC: 1:50-1:100
Western blot analysis of MMP9 on rat spleen tissue lysate using anti-MMP9 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MMP9 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-MMP9 antibody. Counter stained with hematoxylin.
ICC staining MMP9 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining MMP9 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of A431 cells with MMP9 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
背景
The matrix metalloproteinases (MMPs) are a family of peptidase pathway responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin MMP-9 (also designated 92 kDa type IV collagenase or gelatinase B) has been shown to degrade bone collagens in concert with MMP-1 (also specified interstitial collagenase, fibroblast collagenase or Collagenase-1), and cysteine proteases and may play a role in bone osteoclastic resorption. MMP-1 is downregulated by p53, and abnormality of p53 expression can contribute to joint degradation in rheumatoid arthritis by regulating MMP-1 expression.
背景文献
1. Xu L et al. Umbilical cord-derived mesenchymal stem cells on scaffolds facilitate collagen degradation via upregulation of MMP-9 in rat uterine scars. Stem Cell Res Ther 8:84 (2017).
2. Gong L et al. Transthyretin regulates the migration and invasion of JEG-3 cells. Oncol Lett 13:1242-1246 (2017).
Yes
