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位置: 首页 > ELISA试剂盒 > Human Lipoxin A4 (LXA4) ELISA Kit

Human Lipoxin A4 (LXA4) ELISA Kit#EK12146

是否有货: 1-2周
产品总价:
产品详情

产品名称Human Lipoxin A4 (LXA4) ELISA Kit

简述ELISA Kit

应用ELISA

种属反应性Human (Homo sapiens)

保存The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37C for 4 and 7 days, and compare O.D.values of the kit kept at 37C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37C can be considered as 6 months at 2 - 8C, which means 7 days at 37C equaling 12 months at 2 - 8C).

应用详情
Detect Range:493.8-40000 pg/mL
Sensitivity:175.4 pg/mL
Sample Type:Serum, Plasma, Other biological fluids
Sample Volume: 1-200 μL
Assay Time:1-4.5h
Detection wavelength:450 nm
产品描述
Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate LXA4 in samples. An antibody specific for LXA4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLXA4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LXA4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LXA4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Lipoxins are a series of anti-inflammatory mediators. Lipoxins are short lived endogenously produced nonclassic eicosanoids whose appearance in inflammation signals the resolution of inflammation. They are abbreviated as LX, an acronym for lipoxygenase (LO) interaction products. At present two lipoxins have been identified; lipoxin A4?() and lipoxin B4?(LXB4).Lipoxins, as well as certain peptides, are high affinity ligands for the lipoxin A4?receptor (LXA4R), which was first identified based on sequence homology as the formyl peptide receptor like receptor (FPRL1). Lipoxin signaling through the LXA4R inhibits chemotaxis, transmigration, superoxide generation and NF-kappa B activation. Similarly to the leukotrienes, LXA4?will form the cysteinyl-lipoxins LXC4, LXD4?and LXE4. At subnanomolar concentrations, LXA4 and LXB4 inhibit leukotriene-stimulated interactions of human neutrophils and endothelial cells.

如果您使用该产品EK12146发表了文章,请通知我们,让我们可以引用您的文献。

et al,Prognostic impacts of Lipoxin A4 in patients with acute myocardial infarction: A prospective cohort studyInPharmacol ResOn2023 JanbyRunzhen Chen , Jiannan Li et al..PMID:36549409, , (2023),
PMID: 36549409
et al,Plasma Lipid Mediators Associate With Clinical Outcome After Successful Endovascular Thrombectomy in Patients With Acute Ischemic Stroke. In Front Immunol on 2022 Jul 4 by Jiheng Hao, Yao Feng,et al..PMID:35865524, , (2022),
PMID: 35865524
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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