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位置: 首页 > 磷酸化抗体 > HSP27(Phospho-Ser15) Antibody

HSP27(Phospho-Ser15) Antibody#11164

HSP27(Phospho-Ser15) Antibody
HSP27(Phospho-Ser15) Antibody
HSP27(Phospho-Ser15) Antibody
HSP27(Phospho-Ser15) Antibody
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产品总价:
产品详情

产品名称HSP27(Phospho-Ser15) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC IF

种属反应性Hu

特异性The antibody detects endogenous level of HSP27only when phosphorylated at serine 15.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine15 (G-P-S(p)-W-D) derived from Human HSP27.

基因/蛋白名称HSP27

修饰Phospho

别名CMT2F, HMN2B, HSP27, HSP28, Hsp25

数据库入口号Swiss-Prot: P04792
NCBI Protein: NP_001531.1

UniprotP04792

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 27kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from Hela cells untreated or treated with UV using HSP27(Phospho-Ser15) Antibody #11164.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27(Phospho-Ser15) Antibody #11164(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic staining using HSP27(Phospho-Ser15) Antibody #11164.
Western blot analysis of extracts from Hela cells, treated with Anisomycin or calf intestinal phosphatase (CIP), using HSP27 (Phospho-Ser15) Antibody #11164.
背景

Involved in stress resistance and actin organization.

Tremolada L, et al. (2005) Proteomics. 5(3): 788-795.
McGregor E, et al. (2004) Mol Cell Proteomics. 3(2): 115-124.
Benn SC, et al. (2002) Neuron. 36(1): 45-56.
MacDonald JA, et al. (2002) Mol Cell Proteomics. 1(4): 314-322.

如果您使用该产品11164发表了文章,请通知我们,让我们可以引用您的文献。

Ah-Mee Park a, MasatoshiKudo b, SatoruHagiwara b et al,p38MAPK suppresses chronic pancreatitis by regulating HSP27 and BAD expression, Free Radical Biology and Medicine, 52(11-12):2284-91 (2012),
PMID: 22549003
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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