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位置: 首页 > 重组蛋白 > SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein (His tag)

SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein (His tag)#AP89519

是否有货: Yes
产品总价:询价
产品详情

产品名称SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein (His tag)

简述Recombinant Proteins

纯化> 90 % as determined by SDS-PAGE.

生物活性Testing in progress

内毒素< 1.0 EU per ug protein as determined by the LAL method.

免疫原描述A DNA sequence encoding the NCP-CoV(2019-nCoV) Nucleocapsid Protein was expressed with a polyhistidine tag at the C-terminus.

别名coronavirus NP Protein, 2019-nCoV; coronavirus Nucleocapsid Protein, 2019-nCoV; coronavirus Nucleoprotein Protein, 2019-nCoV; cov np Protein, 2019-nCoV; ncov NP Protein, 2019-nCoV; NCP-CoV Nucleocapsid Protein, 2019-nCoV; novel coronavirus NP Protein, 2019-nCoV; novel coronavirus Nucleocapsid Protein, 2019-nCoV; novel coronavirus Nucleoprotein Protein, 2019-nCoV; np Protein, 2019-nCoV; nucleocapsid Protein, 2019-nCoV; Nucleoprotein Protein, 2019-nCoV

计算分子量47.08 kDa.

配方Lyophilized from sterile 20mM Tris, 500mM NaCl, pH8.0, 10%glycerol Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

保存Samples are stable for up to twelve months from date of receipt at -20˚C to -80˚CStore it under sterile conditions at -20˚C to -80˚C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

产品描述

Predicting N End: Met
Shipping Method: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

背景

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

背景文献
1.Van Boheemen S, et al. (2012), MBio. 3(6):e00473-12.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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