纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
种属反应性Hu Ms Rt
特异性The antibody detects endogenous level of total Tau protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa. 402~406 (D-T-S-P-R) derived from Human Tau.
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 48 62 78 kd
Western blotting: 1:500~1:1000
背景
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Li G, Yin H, et.al .(2004) J Biol Chem ; 279(16): 15938-45.
Noble W, et al. (2003) Neuron ; 38(4): 555-65.
Giasson BI, et al.(2002). Biochemistry; 41(51): 15376-87.
Lee G., et.al. (1989). Neuron 2:1615-1624.
Andreadis A.et.al. (1992) Biochemistry 31:10626-10633.
Yes
英文版
