免费电话:400-9961-933
邮箱:techcn@signalwayantibody.com
欢迎光临本站
登录 注册 English
位置: 首页 > ELISA试剂盒 > Human IL-8 ELISA kit

Human IL-8 ELISA kit#EK0453

Human IL-8 ELISA kit
是否有货: 1-2周
产品总价:
产品详情

产品名称Human IL-8 ELISA kit

简述ELISA Kit

应用ELISA

种属反应性Hu

特异性Natural and recombinant Human IL-8 Ligand

交叉反应性No significant interference observed with available related molecules.

基因/蛋白名称Human IL-8

应用详情
Detect Range: 15.625-1000pg/ml
Sensitivity: 7pg/mL
Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume: 20 uL
Assay Time: 3 hours
Detection method: Colorimetric
Representative standard curve for IL-8 ELISA. IL-8 was diluted in serial two-fold steps in Sample Diluent.
产品描述
  • Aluminium pouches with a Microwell Plate coated with antibody to human IL-8 (8 x12)
  • 1 vials human IL-8 Standard lyophilized, 2000 pg/ml upon reconstitution
  • 1 vials concentrated Biotin-Conjugate anti-human IL-8 antibody
  • 1 vials Streptavidin-HRP solution
  • 1 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 2 pieces Adhesive Films
  • package insert
背景

Interleukin 8 (IL-8), a member of the neutrophil-specific CXC subfamily of chemokines, is a potent neutrophil chemotactic and activating factor. It is a primary inflammatory cytokine produced by many cells including monocytes/ macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, astrocytes and chondrocytes. It is in response to proinflammatory stimuli such as IL-1, TNF, LPS and viruses. Its function is, in part, to attract neutrophils to the site of inflammation and to activate them(1-6).

The human IL-8 cDNA sequence predicts a protein of 99 amino acids. Removal of a 22-residue signal peptide generates a mature protein of 77 amino acids (~ 8 kDa). Further proteolysis of the N-terminal end leads to a variant form with 72 amino acids; full activation of IL-8 may require cleavage to the 72 amino acid form.

IL-8 can form non-covalent dimers in solution, especially at high concentrations, but dimerization is not necessary for biological activity. IL-8 binds to two seven-transmembrane, G protein-coupled receptors, CXCR1 and CXCR2, as well as to the non-signalling Duffy antigen on red-blood cells. The Duffy antigen may play a role in regulating IL-8 activity on functional receptors.

背景文献

Oppenheim, J.J. (1991) Annu. Rev. Immunol. 9:617.

Miller, M.D. and M.S. Krangel (1992) Crit. Rev. Immunol. 12:17.

Matsushima, K. et al. (1992) Chem. Immunol. 51:236.

Taub, D.D. and J.J. Oppenheim (1993) Cytokine 5:175.

Vaddi, K. et al. (1997) The Chemokine Facts Book, Academic Press, p. 23.

Hack, C.E. et al. (1997) Adv. Immunol. 66:101.

如果您使用该产品EK0453发表了文章,请通知我们,让我们可以引用您的文献。

et al,Overexpression of CD64 on CD14++CD16? and CD14++CD16+ monocytes of rheumatoid arthritis patients correlates with disease activity.In Exp Ther Med. On 2018 Sep By Luo Q, Xiao P et al..PMID: 30210612, , (2018),
PMID: 30210612
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

联系我们

Location :

南京市高新开发区星火路10号人才大厦C座

Phone :

400-9961-933
025-58868422

Email Address

techcn@signalwayantibody.com