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位置: 首页 > ELISA试剂盒 > Mouse IL-4 ELISA kit

Mouse IL-4 ELISA kit#EK0498

Mouse IL-4 ELISA kit
是否有货: 1-2周
产品总价:
产品详情

产品名称Mouse IL-4 ELISA kit

简述ELISA Kit

应用ELISA

种属反应性Ms

特异性Natural and recombinant Mouse IL-4 Ligand

交叉反应性No significant interference observed with available related molecules.

基因/蛋白名称Mouse IL-4

应用详情
Detect Range: 0.03 - 2.0 ng/mL
Sensitivity: 15pg/mL
Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume: 20 uL
Assay Time: 3 hour
Detection method: Colorimetric
Representative standard curve for IL-4 ELISA. IL-4 was diluted in serial two-fold steps in Sample Diluent.
产品描述
  • Aluminium pouches with a Microwell Plate coated with antibody to mouse IL-4 (8X12)
  • 2 vials mouse IL-4 Standard lyophilized, 2000 pg/ml upon reconstitution
  • 2 vials concentrated Biotin-Conjugate anti-mouse IL-4 antibody
  • 2 vials Streptavidin-HRP solution
  • 1 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 4 pieces Adhesive Films
  • package insert
背景

Interleukin 4 (IL-4) is a pleiotropic cytokine produced primarily by activated T lymphocytes, mast cells and basophils (1-3).

The cDNA sequence of mouse IL-4 predicts a 140 amino acid (aa) residue precursor protein containing a 20 aa residue signal peptide that is cleaved to form the mature protein (4). At the amino acid sequence level, mature mouse IL-4 is approximately 50% identical to human IL-4 but there is no species cross-reactivity for biological activity for the two proteins (1, 2). Mouse IL-4 also shares approximately 30% amino acid sequence identity to mouse IL-13 and the two cytokines exhibit overlapping biological activities (5, 6). The gene for IL-4 has been mapped to mouse chromosome 11, in close proximity to the genes for IL-3, IL-5, IL-13 and GM-CSF (1, 2).

IL-4 has multiple immune response-modulating activities on a variety of cell types. It is an important regulator of isotype switching, inducing IgE production in B lymphocytes. It is an important modulator of the differentiation of precursor T helper cells to the Th2 subset that mediates humoral immunity and modulates antibody production. In addition, IL-4 has also been shown to have anti-tumor activity both in vivo and in vitro (1-3).

The biological effects of IL-4 are mediated by specific cell surface receptor complexes. Although IL-4 R does not bind IL-13 directly, it has been shown to complex with the low-affinity IL-13 R to form the functional high-affinity receptor complex for IL-13 (7, 8). In addition to the membrane-bound form of IL-4 R, a naturally occurring soluble form of IL-4 R has been identified in human and mouse biological fluids and in mouse cell culture supernates (9-11). Soluble IL-4 R has been to shown to bind IL-4 with high affinity in solution.

背景文献

Howard, M. and N. Harada (1994) in Guidebook to Cytokines and Their Receptors,Nicola N.A. ed., Oxford University Press, New York, p. 44.

Banchereau, J. and M.E. Rybak (1994) in The Cytokine Handbook, 2nd ed., A. Thomson ed., Academic Press, New York, p. 99.

Yokota, T. et al. (1990) in Peptide Growth Factors and Their Receptors I, Sporn, M.B. And A.B. Roberts eds., Springer-Verlag, New York, p. 577.

Lee, F. et al. (1986) Proc. Natl. Acad. Sci. USA 83:2061.

McKenzie, A.N.J. and G. Zurawski (1994) in Guidebook to Cytokines and Their Receptors, Nicola N.A. ed., Oxford Univ. Press, New York, p. 92.

Zurawski, G. and J.E. de Vries (1994) Immunol. Today 15:19.

Lin, J-X. et al. (1995) Immunity 2:331.

Zurawski, S.M. et al. (1995) J. Biol. Chem. 270:13869.

Mosley, B. et al. (1989) Cell 59:335.

Fernandez-Botran, R. and E.S. Vitetta (1990) Proc. Natl. Acad. Sci. USA 87:535.

Fanslow W.C. et al. (1990) Cytokine 2:3028.

如果您使用该产品EK0498发表了文章,请通知我们,让我们可以引用您的文献。

et al,Immune and Metabolic Regulation Mechanism of Dangguiliuhuang Decoction against Insulin Resistance and Hepatic Steatosis.In Front Pharmacol on 2017 Jul 7 by Hui Cao , Lingling Tuo,et al..PMID: 28736524, , (2017),
PMID: 28736524
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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