产品详情
产品名称Thor Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用Custom antibody
种属反应性Fruit fly
免疫原类型Peptide-KLH
基因/蛋白名称Thor
别名Eukaryotic translation initiation factor 4E-binding protein 4E-BP 4E-binding protein Thor dThor
数据库入口号uniprot:Q9XZ56
计算分子量13KDa
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 60% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Western blotting: 1:500~1:1000,
Immunohistochemistry: 1:50~1:110
背景
Repressor of translation initiation that regulates eIF4E1 activity by preventing its assembly into the eIF4F complex (PubMed:11389445, PubMed:19804760, PubMed:25702871). Hypophosphorylated form competes with eIF4G1 and strongly binds to eIF4E1, leading to repress translation (PubMed:25702871). In contrast, hyperphosphorylated form dissociates from eIF4E1, allowing interaction between eIF4G1 and eIF4E1, leading to initiation of translation (PubMed:25702871). Acts as a regulator of various biological processes, such as innate immunity, cell growth or synaptic transmission (PubMed:10811906, PubMed:11389445, PubMed:27525480). Acts downstream of phosphoinositide-3-kinase (PI3K) to regulate cell growth (PubMed:11389445). Extends lifespan upon dietary restriction by regulating the mitochondrial translation (PubMed:19804760). Acts as a regulator of lifespan in response to cold by regulating the mitochondrial translation (PubMed:28827349). Acts as a negative regulator of presynaptic release of neurotransmitter in motor neurons: Thor expression is induced in response to insulin signaling, leading to prevent of translation of complexin (cpx), a protein known to regulate the exocytosis of synaptic vesicles (PubMed:27525480). Acts as a negative regulator of synaptic strength at the neuromuscular junction: Thor expression in response to acute fasting prevents translation, thereby suppressing retrograde synaptic enhancement (PubMed:27916456).
Yes
