产品详情
产品名称Goat lmmunoglobulin A (IgA) ELISA Kit
简述ELISA Kit
应用ELISA
种属反应性Goat
保存The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37C for 4 and 7 days, and compare O.D.values of the kit kept at 37C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37C can be considered as 6 months at 2 - 8C, which means 7 days at 37C equaling 12 months at 2 - 8C).
应用详情
Detect Range:0.78 ??g/mL - 50 ??g/mL
Sensitivity:0.39 ??g/mL
Sample Type:Serum, Plasma, Other biological fluids
Sample Volume: 1-200 ??L
Assay Time:1-4.5h
Detection wavelength:450 nm
产品描述
This assay employs a two-site sandwich ELISA to quantitate IgA in Goat serum, plasma, breast milk. An antibody specific for IgA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IgA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IgA bound in the initial step. The color development is stopped and the intensity of the color is measured.
2-3 weeks
