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位置: 首页 > 磷酸化抗体 > Histone H2A.X(Phospho-Ser139) Antibody

Histone H2A.X(Phospho-Ser139) Antibody#11268

Histone H2A.X(Phospho-Ser139) Antibody
Histone H2A.X(Phospho-Ser139) Antibody
Histone H2A.X(Phospho-Ser139) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称Histone H2A.X(Phospho-Ser139) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IF

种属反应性Hu

特异性The antibody detects endogenous level of Histone H2A.X only when phosphorylated at serine 139.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 139 (Q-A-S(p)-Q-E) derived from Human Histone H2A.X.

基因/蛋白名称Histone H2A.X

修饰Phospho

别名H2A.X; H2AFX; H2a/x; HIST5-2AX;

数据库入口号Swiss-Prot: P16104
NCBI Protein: NP_002096.1

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 15kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from HT29 cells untreated(lane 1) or treated with UV(lane 2) using Histone H2A.X(Phospho-Ser139) Antibody #11268.
Immunofluorescence staining of methanol-fixed Hela cells using Histone H2A.X(Phospho-Ser139) Antibody #11268.
Western blot analysis of extracts from 293 cells, treated with UV+serum or calf intestinal phosphatase (CIP), using Histone H2A.X (Phospho-Ser139) Antibody #11268.
背景

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Yaneva M, et al. (2005) Nucleic Acids Res. 33(16): 5320-5330.
Tsukuda T, et al.(2006) Nature. Author manuscript; available in PMC 2006 March 6.

如果您使用该产品11268发表了文章,请通知我们,让我们可以引用您的文献。

et al,T 2 toxin cytotoxicity mediated by directly perturbing mitochondria in human gastric epithelium GES 1 cells.In J Appl Toxicol on 2020 Mar 18. by Su N, Liu CL, et al..PMID:32187393, , (2020),
PMID: 32187393
et al,Jaridonin-induced G2/M Phase Arrest in Human Esophageal Cancer Cells Is Caused by Reactive Oxygen Species-Dependent Cdc2-tyr15 Phosphorylation via ATM-Chk1/2-Cdc25C Pathway .In Toxicol Appl Pharmacol on 2015 Jan 15 by Yong-Cheng Ma , Nan Su et al..PMID:25450480, , (2015),
PMID: 25450480
Xiukun Cu, Jing Zhang, Rong Du et al,HSF4 is involved in DNA damage repair through regulation of Rad51, Biochimica et Biophysica Acta, 1822(8):1308篓C1315 (2012),
PMID: 22587838
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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