产品详情
产品名称PKM2(phospho-Ser37) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB IF
种属反应性Hu
特异性The antibody detects endogenous level of PKM2 only when phosphorylated at serine 37.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of serine 37(I-D-S(p)-P-P) derived from Human PKM2.
基因/蛋白名称PKM2
修饰Phospho
别名PKM, PK3, OIP3, PK2
数据库入口号Swiss-Prot#: P14618 NCBI Protein#: NP_872270.1
UniprotP14618
实际分子量60kd
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C
应用详情
Predicted MW: 60kd
Western Blot: 1:500~1:1000
Immunofluorescence: 1:100~1:200
背景
Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
Weiwei Yang1, Yanhua Zheng1, Yan Xia1, Haitao Ji, "ERK1/2-dependent phosphorylation and nuclear translocation of PKM2 promotes the Warburg effect." Nature Cell Biology(2012)|doi:10.1038/ncb2629
Received16 August 2012 Accepted24 October 2012 Published online25 November 2012
如果您使用该产品11456发表了文章，请通知我们，让我们可以引用您的文献。
et al,ATM inhibition blocks glucose metabolism and amplifies the sensitivity of resistant lung cancer cell lines to oncogene driver inhibitors InCancer MetabOn2023 Nov 6byCristina Terlizzi , Viviana De Rosa et al..PMID:37932830,
, (2023),
PMID: 37932830
et al,Warburg-like metabolic transformation underlies neuronal degeneration in sporadic Alzheimer's disease.In Cell Metab. 2022 Sep 6 by Larissa Traxler 1, Joseph R Herdy,et al..PMID: 35987203,
, (2022),
PMID: 35987203
et al,LncCCAT1 interaction protein PKM2 upregulates SREBP2 phosphorylation to promote osteosarcoma tumorigenesis by enhancing the
Warburg effect and lipogenesis. In Int J Oncol on 2022 Apr by Feifei Pu, Jianxiang Liu,et al..PMID:35244192,
, (2022),
PMID: 35244192
et al,Oncogenic TRIB2 interacts with and regulates PKM2 to promote aerobic glycolysis and lung cancer cell procession. In Cell Death Discov on 2022 Jul 5 by Yuan-Rong Liu, Dan-Dan Song, et al..PMID:35790734,
, (2022),
PMID: 35790734
et al,TRIM35-mediated degradation of nuclear PKM2 destabilizes GATA4/6 and induces P53 in cardiomyocytes to promote heart failure. In Sci Transl Med
on 2022 Nov 2 by Maria Areli Lorenzana-Carrillo, Keshav Gopal,et al..PMID: 36322626,
, (2022),
PMID: 36322626
et al,Phosphoproteome of signaling by ErbB2 in ovarian cancer cells. In Biochim Biophys Acta Proteins Proteom on 2022 Apr 1 by C Sidhanth, S Bindhya,et al..PMID:35158093,
, (2022),
PMID: 35158093
et al,Non-Canonical Role of PDK1 as a Negative Regulator of Apoptosis through Macromolecular Complexes Assembly at the ER锟紺Mitochondria Interface in Oncogene-Driven NSCLC.
In Cancers (Basel) on 2021 Aug 17 by Viviana De Rosa, Francesca Iommelli,et al..PMID:34439291,
, (2021),
PMID: 34439291
查看更多
et al,Estrogen activates pyruvate kinase M2 and increases the growth of TSC2-deficient cells.In PLoS One on 2020 Feb 20; by Lu Y, Liu X, et al..PMID: 32078667,
, (2020),
PMID: 32078667
et al,Exploring rapamycin-induced pro-survival pathways in Tuberous Sclerosis Complex and the development of alternative therapies,
, (2020),
PMID:
et al,Nuclear translocation of PKM2/AMPK complex sustains cancer stem cell populations under glucose restriction stress.In Cancer Lett. On 2018 May 1 et al..PMID:29408265 ,
, (2018),
PMID: 29408265
et al,Cross-Talk Between Alternatively Spliced UGT1A Isoforms and Colon Cancer Cell Metabolism.In Mol Pharmacol on 2017 Mar by Yannick Audet-Delage,Mich猫le Rouleau,et al..PMID: 28049773,
, (2017),
PMID: 28049773
et al,Glut1 Promotes Cell Proliferation, Migration and Invasion by Regulating Epidermal Growth Factor Receptor and Integrin Signaling in Triple-Negative Breast Cancer Cells.In BMB Rep on 2017 Mar by Sunhwa Oh, Hyungjoo Kim,et al..PMID: 27931517,
, (2017),
PMID: 27931517
et al,Selective GPR55 Antagonism Reduces Chemoresistance in Cancer Cells .In Pharmacol Res on 2016 Sep by Nagendra S Singh , Michel Bernier et al..PMID: 27423937,
, (2016),
PMID: 27423937
et al,Vitamin C uncouples the Warburg metabolic switch in KRAS mutant colon cancer.In Oncotarget on 2016 Jul 26 by Oscar Aguilera , Mar铆a Mu帽oz-Sagastibelza et al..PMID:27323830 ,
, (2016),
PMID: 27323830
et al,PKM2 dephosphorylation by Cdc25A promotes the Warburg effect and tumorigenesis.In Nat Commun on 2016 Aug 3 by Ji Liang , Ruixiu Cao et al..PMID: 27485204,
, (2016),
PMID: 27485204
et al,ECM1 promotes the Warburg effect through EGF-mediated activation of PKM2.In Cell Signal on 2015 Feb by Kyung-Min Lee, Keesoo Nam et al ..PMID: 25446258,
, (2015),
PMID: 25446258
et al,Estradiol-17尾 Upregulates Pyruvate Kinase M2 Expression to Coactivate Estrogen Receptor-伪 and to Integrate Metabolic Reprogramming With the Mitogenic Response in Endometrial Cells .In J Clin Endocrinol Metab on 2014 Oct by Salama A Salama, Mahmoud A Mohammad et al..PMID:24471565,
, (2014),
PMID: 24471565
et al,ERK1/2-dependent phosphorylation and nuclear translocation of PKM2 promotes the Warburg effect.In Nat Cell Biol on 2012 Dec by
Weiwei Yang, Yanhua Zheng,et al..PMID:23178880,
, (2012),
PMID: 23178880
有货
