PI3-kinase p85- alpha (Phospho-Tyr607) Antibody#12057
评价
有货
All Lanes: PI3-kinase p85- (Phospho-Tyr607) Antibody at 1/ 500 dilution.
Lane1: MCF7 serum starvation
Lane2: MCF7 treated with 20nM EGF for 15min
Lane3:MCF7 treated with 100nM Dasatinib for 30min
Lane4: MCF7 Serum Starvation, then treated with Lambda Protein Phosphatase
Lane5: MCF7 treated with 20nM EGF for 15min, then treated with Lambda Protein Phosphatase
Lane6:MCF7 treated with 100nM Dasatinib for 30min, then treated with Lambda Protein Phosphatase Lysates/ Proteins at 40ug per lane
Secondary: Goat Anti-Rabbit IgG(HRP) at 1/20000 dilution
Predicted band size : 80kDa
Observed band size: 80kDa
Lane1: Rat kidney tissue lysate Lane2: Rat kidney tissue lysate, blocked with the phospho peptide
Immunohistochemical analysis of paraffin-embedded Human placenta. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Immunohistochemical analysis of paraffin-embedded Mouse-colon tissue. 1,PI 3-kinase p85α (phospho Tyr607) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1,PI 3-kinase p85α (phospho Tyr607) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1,PI 3-kinase p85α (phospho Tyr607) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemistry analysis of paraffin-embedded human brain, using PI3-kinase p85-alpha (Phospho-Tyr607) Antibody. The picture on the right is blocked with the phospho peptide.
