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位置: 首页 > 磷酸化抗体 > Bub3 (Phospho-Tyr207) Antibody

Bub3 (Phospho-Tyr207) Antibody#11586

Bub3 (Phospho-Tyr207) Antibody
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产品总价:
产品详情

产品名称Bub3 (Phospho-Tyr207) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of Bub3 only when phosphorylated at Tyrosine 207.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of Tyrosine 207 (V-E-Y(p)-L-D) derived from Human Bub3.

基因/蛋白名称Bub3

修饰Phospho

别名Mitotic checkpoint protein BUB3, Hbub3, BUB3L, BUB3 budding uninhibited by benzimidazoles 3 homolog, budding uninhibited by benomyl

数据库入口号Swiss-Prot#: O43684 NCBI Protein#: NP_001007794.1

UniprotO43684

实际分子量40KD

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C/1 year

应用详情

 Western blotting: 1:1000

Western blot analysis of Bub3 (Phospho-Tyr207)Antibody #11586 (arrow indicated) in U87 (A) and U87 EGFRvIII (B) cells in Mitosis.
背景
Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.
 
 
Seeley T.W., Wang L., Zhen J.Y.Biochem. Biophys. Res. Commun. 257:589-595(1999)
Tang Z., Shu H., Oncel D., Chen S., Yu H.Mol. Cell 16:387-397(2004)
Logarinho E., Resende T., Torres C., Bousbaa H.Mol. Biol. Cell 19:1798-1813(2008)
 

如果您使用该产品11586发表了文章,请通知我们,让我们可以引用您的文献。

et al,PKM2 regulates chromosome segregation and mitosis progression of tumor cells.In Mol Cell on 2014 Jan 9 by Yuhui Jiang, Xinjian Li et al..PMID: 24316223, , (2014),
PMID: 24316223
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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