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位置: 首页 > 单克隆抗体 > EGFP/EYFP Monoclonal Antibody

EGFP/EYFP Monoclonal Antibody#27209

EGFP/EYFP Monoclonal Antibody
EGFP/EYFP Monoclonal Antibody
EGFP/EYFP Monoclonal Antibody
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产品总价:
产品详情

产品名称EGFP/EYFP Monoclonal Antibody

来源种属Mouse

克隆性Monoclonal

克隆性3F11-B12-E7-F12

亚型IgG1

纯化Affinity purified

应用WB

种属反应性Bacillus cereus

特异性Transfected

免疫原类型Recombinant Protein

免疫原描述Purified recombinant EYFP.tag full length expressed in E.coli.

基因/蛋白名称EGFP/EYFP

别名GFP; Green Fluorescent Protein; enhanced Green Fluorescent Protein;

数据库入口号Uniprot: C8CHS1

UniprotC8CHS1

实际分子量According

配方Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.

保存store at -20˚C

应用详情

 Western blotting: 1:1000

Western blot detection of EGFP expression in 293T cells transfected (A:mRFP) or transfected (B:EGFP fused protein;C:EGFP) using EGFP antibody(L:1/60000 diluted. M:1/20000 diluted. R:1/10000 diluted).
Western blot detection of EYFP expression in Rosetta E.coli cells induced by 0(A)or 0.1mM (B)IPTG using EGFP antibody(1:1000 diluted).Predicted band size: 30KDa Observed band size: 30KDa.
Western blot detection of EGFP expression in Hela cells non-transfected (A) or transfected (B) with pEGFP C1 using EGFP antibody(1:5000 diluted).Predicted band size: 34KDa Observed band size: 34KDa.
背景

 The green fluorescent protein (GFP) is a protein isolated from the jellyfish Aequorea victoria composed of 238 amino acid residues that exhibits bright green fluorescence. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineages and defines subcellular protein localizations. Due to the potential for widespread usage and the evolving needs of researchers, many different mutants of GFP have been engineered. For example, EGFP contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr which result in  brighter green fluorescence. EYFP contains the four amino acid substitutions of Ser-65 to Gly; Val-68 to Leu; Ser-72 to Ala; and Thr-203 to Tyr. EYFP emits yellow fluorescence excited by green light.

如果您使用该产品27209发表了文章,请通知我们,让我们可以引用您的文献。

et al,Purification and characterization of a novel cell-penetrating carrier similar to cholera toxin chimeric protein.In Protein Expr Purif on 2017 Jan by Weiping Lin, Xi Zheng ,et al..PMID: 27234002, , (2017),
PMID: 27234002
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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