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Ephrin-B2(Ab-330) Antibody#21196

Ephrin-B2(Ab-330) Antibody
Ephrin-B2(Ab-330) Antibody
Ephrin-B2(Ab-330) Antibody
Ephrin-B2(Ab-330) Antibody
Ephrin-B2(Ab-330) Antibody
Ephrin-B2(Ab-330) Antibody
Ephrin-B2(Ab-330) Antibody
Ephrin-B2(Ab-330) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称Ephrin-B2(Ab-330) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antigen affinity purification

应用IF,WB,IHC,ELISA

种属反应性Human, Mouse

特异性The antibody detects endogenous level of total Ephrin-B2 protein.

免疫原类型Peptide-KLH

免疫原描述Synthetic peptide of human EFNB2

基因/蛋白名称Ephrin-B2

别名EFNB2; HTKL; LERK5

数据库入口号Swiss-Prot: P52799
NCBI Protein: NP_004084.1

浓度0.8 mg/ml

配方pH7.4 PBS, 0.05% NaN3, 40% Glycerol

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

WB: 1:500~1:2000
IHC: 1:50~1:200
IF:1:20~1:50

21196-lot 7326 at 1/200 staining human renal clear cell carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
21196-lot 7326 at 1/200 staining human meningeal carcinomatosis(MC) tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary
21196-lot 7326 at 1/100 staining mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary
21196-lot 7326 at 1/100 staining rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Immunofluorescent analysis of formalin-fixed, paraffin-embedded hela cells with 21196-lot 7326 at 1/75 dilution. A Goat Anti-Rabbit IgG H&L (CY3) at 1/50 was used as secondary.
Immunofluorescent analysis of formalin-fixed, paraffin-embedded C6 cells with 21196-lot 7326 at 1/75 dilution. A Goat Anti-Rabbit IgG H&L (CY3) at 1/50 was used as secondary.
Gel: 8% SDS-PAGELysate: 40 μgLane: A549 cell lysatePrimary antibody: Ephrin-B2(Ab-330) Antibody at dilution 1/800Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilutionExposure time: 10 seconds
All Lanes: Ephrin-B2(Ab-330) Antibody at 1/1000 dilution.Lane1: 293 cells treated with 20ng/ml TNF-a for 30minLane2: 293 cells blocked with the phospho peptide.Lysates/ Proteins at 60ug per lane Secondary: Goat Anti-Rabbit IgG(HRP) at 1/20000 dilutionPredicted band size : 37kDaObserved band size: 57kDa
背景

Ephrin-B2 encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a lycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNB class ephrin which binds to the EPHB4 and EPHA3 receptors.

Chrencik JE,et al.( 2006) J Biol Chem;281(38):28185-28192.
Kertesz N, et al. (2006) Blood; 107(6):2330-2338.
Noren NK, et al. (2004) Proc Natl Acad Sci USA; 101(15):5583-5588.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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