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位置: 首页 > 磷酸化抗体 > GSK3β(Phospho-Ser9) Antibody

GSK3β(Phospho-Ser9) Antibody#11002

GSK3β(Phospho-Ser9) Antibody
GSK3β(Phospho-Ser9) Antibody
GSK3β(Phospho-Ser9) Antibody
GSK3β(Phospho-Ser9) Antibody
GSK3β(Phospho-Ser9) Antibody
GSK3β(Phospho-Ser9) Antibody
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产品总价:
产品详情

产品名称GSK3β(Phospho-Ser9) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC IF

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of GSK3 beta only when phosphorylated at serine 9.

免疫原类型Peptide-KLH

免疫原描述Synthesized phospho-peptide around the phosphorylation site of human GSK3β (phospho Ser9)

基因/蛋白名称GSK3β

修饰Phospho

别名Factor A, GSK-3 beta, Protein kinase GSK-3-beta, kinase GSK-3 beta

数据库入口号Swiss-Prot: P49841
NCBI Protein: NP_x.1

UniprotP49841

实际分子量46kd

浓度1.0mg/ml

配方Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

保存Store at -20°C for long term preservation (recommended). Avoid Store at 4°C for short term use.

应用详情

Predicted MW: 46kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from 293 cells untreated(lane 1) or treated with serum(lane 2) using GSK3β(Phospho-Ser9) Antibody #11002.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3β (Phospho-Ser9) Antibody #11002 (left) or the same antibody preincubated with blocking peptide #51002 (right).
Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic staining using GSK3β(Phospho-Ser9) Antibody #11002.
Immunohistochemical analysis of paraffin-embedded human Lung carcinoma tissue using GSK3β (Phospho-Ser9) Antibody #11002.
Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic staining using GSK3β (Phospho-Ser9) Antibody #11002.
Western blot analysis of extracts from Hela cells, treated with EGF or calf intestinal phosphatase (CIP), using GSK3β (Phospho-Ser9) Antibody #11002.
背景

Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin.

Fan G, et al. (2003) J Biol Chem. 278(52): 52432-52436.
Barry FA, et al. (2003) FEBS Lett. 553(1-2): 173-178.
Welsh, et al. (1996) Trends Cell Biol. 6: 274-279.
Srivastava A K, et al. (1998) Mol Cell Biochem. 182: 135-141.

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Yu-fei Pan, Li-wei Dong, Min Wang et al,Signal regulatory protein 娄脕 negatively regulates mast-cell activation following Fc娄脜RI aggregation., Eur. J. Immunol., 43(6):1598篓C1607 (2013),
PMID: 23504624
et al,Phosphoproteome analysis reveals an important role for glycogen synthase kinase-3 in perfluorododecanoic acid-induced rat liver toxicity. In Toxicol Lett on 2013 Mar 27 by Hongxia Zhang, Junjie Hou, et al..PMID: 23353032, , (2013),
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et al,尾-N-methylamino-l-alanine causes neurological and pathological phenotypes mimicking Amyotrophic Lateral Sclerosis (ALS): The first step towards an experimental model for sporadic ALS. In Environ Toxicol Pharmacol on 2013 Sep by Estefan铆a de Munck, Emma Mu帽oz-S谩ez, et al..PMID: 23688553, , (2013),
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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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