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位置: 首页 > 磷酸化抗体 > c-Jun(Phospho-Ser73) Antibody

c-Jun(Phospho-Ser73) Antibody#11003

c-Jun(Phospho-Ser73) Antibody
c-Jun(Phospho-Ser73) Antibody
c-Jun(Phospho-Ser73) Antibody
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产品总价:
产品详情

产品名称c-Jun(Phospho-Ser73) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of c-Jun only when phosphorylated at serine 73.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 73 (L-A-S(p)-P-E) derived from Human c-Jun.

基因/蛋白名称c-Jun

修饰Phospho

别名AH119; AP1; Jun A; c-Jun; p39

数据库入口号Swiss-Prot: P05412
NCBI Protein: NP_002219.1

UniprotP05412

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 43kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from PC14 cells untreated or treated with EGF using c-Jun(Phospho-Ser73) Antibody #11003.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Jun(Phospho-Ser73) Antibody #11003(left) or the same antibody preincubated with blocking peptide(right).
Western blot analysis of extracts from C6 cells, treated with PMA or calf intestinal phosphatase (CIP), using c-Jun (Phospho-Ser73) Antibody #11003.
背景

Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'.

如果您使用该产品11003发表了文章,请通知我们,让我们可以引用您的文献。

et al,The pivotal role of SUMO-1-JNK-Tau axis in an in vitro model of oxidative stress counteracted by the protective effect of curcumin. In Biochem Pharmacol on 2020 Aug by Lucia Buccarello, Jessica Dragotto, et al..PMID:32502496, , (2020),
PMID: 32502496
et al,Differential apoptosis gene expressions of rhabdomyosarcoma cells in response to enterovirus 71 infection.In BMC Infect Dis on 2012 Nov 28 by Shi W, Li X,et al..PMID:23191987, , (2012),
PMID: 23191987
et al,Increase in claudin-2 expression by an EGFR/MEK/ERK/c-Fos pathway in lung adenocarcinoma A549 cells. In Biochim Biophys Acta on 2012 Jun by Akira Ikari, Tomonari Sato, et al..PMID: 22546605, , (2012),
PMID: 22546605
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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