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EGFR(Ab-869) Antibody#21222

EGFR(Ab-869) Antibody
EGFR(Ab-869) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称EGFR(Ab-869) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

应用WB IHC

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of total EGFR protein.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around aa.867~871 (K-E-Y-H-A) derived from Human EGFR.

基因/蛋白名称EGFR

别名 Receptor tyrosine-protein kinase ErbB-1; ERBB1;

数据库入口号Swiss-Prot: P00533
NCBI Protein: NP_005219.2

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 175kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from HUVEC and MDA cells using EGFR(Ab-869) Antibody #21222.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EGFR(Ab-869) Antibody #21222(left) or the same antibody preincubated with blocking peptide(right).
背景
Receptor for EGF, but also for other members of the EGF family, as TGF-a, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentia

Gingras AC, et al. (1998) Genes Dev 12(4): 502-513.
Brugarolas J, et al. (2004) Genes Dev 18(23): 2893-2904.
Kumar V, et al. (2000) EMBO J 19(5): 1087-1097.
Moody CA, et al. (2005) J Virol 79(9): 5499-5506.
Burnett PE, et al. (1998) Proc Natl Acad Sci U S A 95(4): 1432-1437.

如果您使用该产品21222发表了文章,请通知我们,让我们可以引用您的文献。

et al,Secreted and O-GlcNAcylated MIF binds to the human EGF receptor and inhibits its activation.In Nat Cell Biol on 2015 Oct by Yanhua Zheng, Xinjian Li et al..PMID:26280537 , , (2015),
PMID: 26280537
et al,Aldosterone induces myofibroblast EGF secretion to regulate epithelial colonic permeability. In Am J Physiol Cell Physiol on 2013 May 1 by Llu茂sa Mir贸, Anna P茅rez-Bosque, et al..PMID:23467299 , , (2013),
PMID: 23467299
et al,Role of Proprotein Convertases in Prostate Cancer Progression.In Neoplasia on 2012 Nov by Fr茅d茅ric Couture, Fran莽ois D'Anjou,In Neoplasia on 2012 Nov by Fr茅d茅ric Couture, Fran莽ois D'Anjou, et al..PMID:23226097, , (2012),
PMID: 23226097
et al,EGFR-induced and PKC蔚 monoubiquitylation-dependent NF-魏B activation upregulates PKM2 expression and promotes tumorigenesis. In Mol Cell on 2012 Dec 14 by Weiwei Yang, Yan Xia, et al..PMID: 23123196, , (2012),
PMID: 23123196
et al,sZIP, an alternative splice variant of ZIP, antagonizes transcription repression and growth inhibition by ZIP. In J Biol Chem on 2010 May 7 by Wenhua Yu, Ruifang Li, et al..PMID: 20233718, , (2010),
PMID: 20233718
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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