产品详情
产品名称HMOX1 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were purified by affinity purification using immunogen.
应用WB,IHC,IF
种属反应性Human,Mouse,Rat
特异性The antibody detects endogenous level of total HMOX1 protein.
免疫原类型Recombinant Protein
免疫原描述Recombinant protein of human HMOX1.
基因/蛋白名称HMOX1
别名HO-1; HSP32; bK286B10; HMOX1;
数据库入口号Swiss-Prot:P09601
NCBI Gene ID:3162
UniprotP09601
实际分子量33KD
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20˚C
应用详情
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using HO-1 antibody at 1:1000 dilution.
Immunofluorescence analysis of HeLa cells using HO-1 Polyclonal antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded human liver using HO-1 antibody at dilution of 1:200 (40x lens).
背景
Heme oxygenase (HO) is the rate-limiting enzyme in the catabolism of heme that results in the release of carbon monoxide, iron, and biliverdin (1). The products of this enzymatic reaction play important biological roles in antioxidant, anti-inflammatory and cytoprotective functions (2). Heme oxygenase comprises two isozymes, including the constitutively expressed HO-2 isozyme and the inducible HO-1 isozyme (3). Inducible HO-1 is expressed as an adaptive response to several stimuli, including heme, metals, and hormones (4). The induction of HO-1 has been implicated in numerous disease states, such as transplant rejection, hypertension, atherosclerosis, Alzheimer disease, endotoxic shock, diabetes, inflammation, and neurological disorders (1,5).
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et al,5-(3,4-Difluorophenyl)-3-(6-methylpyridIn-3-yl)-1,2,4-oxadiazole (DDO-7263), a novel Nrf2 activator targetIng braIn tissue, protects agaInst MPTP-Induced subacute ParkInson's disease In mice by InhibitIng the NLRP3 Inflammasome and protects PC12 cells agaInst oxidative stress. In Free Radic Biol Med on 2019 Apr; by Xu LL, Wu YF,et al..PMID:30615919,
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PMID: 30615919