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位置: 首页 > 抗体 > Histone H2A.X(Ab-139) Antibody

Histone H2A.X(Ab-139) Antibody#21260

Histone H2A.X(Ab-139) Antibody
Histone H2A.X(Ab-139) Antibody
Histone H2A.X(Ab-139) Antibody
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产品总价:
产品详情

产品名称Histone H2A.X(Ab-139) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

应用WB IHC IF

种属反应性Hu

特异性The antibody detects endogenous level of total Histone H2A.X protein.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around aa.137~141 (Q-A-S-Q-E) derived from Human Histone H2A.X.

基因/蛋白名称Histone H2A.X

别名H2A.X; H2AFX; H2a/x; HIST5-2AX;

数据库入口号Swiss-Prot: P16104
NCBI Protein: NP_002096.1

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 15kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from HepG2 and 293 cells using Histone H2A.X(Ab-139) Antibody #21260.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Histone H2A.X(Ab-139) Antibody #21260(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using Histone H2A.X(Ab-139) Antibody #21260.
背景
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation

Yaneva M, et al. (2005) Nucleic Acids Res. 33(16): 5320-5330.
Tsukuda T, et al.(2006) Nature. Author manuscript; available in PMC 2006 March 6

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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