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ANGPTL4 Antibody#32550

ANGPTL4 Antibody
ANGPTL4 Antibody
ANGPTL4 Antibody
ANGPTL4 Antibody
ANGPTL4 Antibody
ANGPTL4 Antibody
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产品总价:
产品详情

产品名称ANGPTL4 Antibody

来源种属Rabbit

克隆性Polyclonal

亚型IgG

纯化Affinity purification

应用WB,IHC,IF

种属反应性Human,Mouse,Rat

特异性The antibody detects endogenous level of total ANGPTL4 protein.

免疫原类型Peptide

免疫原描述Recombinant fusion protein of human ANGPTL4 (NP_647475.1).

基因/蛋白名称ANGPTL4

别名ANGPTL4;ARP4;FIAF;HARP;HFARP;NL2;PGAR;TGQTL;UNQ171;pp1158

数据库入口号Uniprot:Q9BY76
GeneID:51129

UniprotQ9BY76

Gene ID51129

实际分子量45KDa

浓度1.0mg/ml

配方PBS with 0.02% sodium azide,50% glycerol,pH7.3.

保存Store at -20˚C. Avoid freeze / thaw cycles.

应用详情

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

All Lanes: ANGPTL4 Antibody at 1/ 500 dilution.Lane1: A549 Cell lysateLane2: 293T Cell lysateLane3: HT29 Cell lysateLane4: HUVEC Cell lysateLane5: Mouse Brain Tissue lysateLane6:Rat lung Tissue lysateLane7:Rat Heart Tissue lysateLysates/proteins at 40 µg per lane. Secondary: Goat Anti-Rabbit IgG(HRP) at 1/20000 dilutionPredicted band size : 45kDaObserved band size: 45kDa
Immunohistochemical analysis of paraffin-embedded Human testis. 1. ANGPTL4 Antibody was diluted at 1:100(4℃ overnight); 2. High-pressure and temperature with EDTA (pH8.0) was used for antigen retrieval. 3. HRP Goat anti-Rabbit IgG Secondary antibody was diluted at 1:200(room temperature, 30min).
Immunofluorescence analysis of NIH/3T3 cells using ANGPTL4 Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry analysis of paraffin-embedded human breast tissue using ANGPTL4 Antibody at dilution 1/150.Negative control was used by secondary antibody only.
Immunohistochemistry analysis of paraffin-embedded mouse testis tissue using ANGPTL4 Antibody at dilution 1/150.Negative control was used by secondary antibody only.
Immunohistochemistry analysis of paraffin-embedded rat testis tissue using ANGPTL4 Antibody at dilution 1/150.Negative control was used by secondary antibody only.
背景

This gene encodes a glycosylated, secreted protein containing a C-terminal fibrinogen domain. The encoded protein is induced by peroxisome proliferation activators and functions as a serum hormone that regulates glucose homeostasis, lipid metabolism, and insulin sensitivity. This protein can also act as an apoptosis survival factor for vascular endothelial cells and can prevent metastasis by inhibiting vascular growth and tumor cell invasion. The C-terminal domain may be proteolytically-cleaved from the full-length secreted protein. Decreased expression of this gene has been associated with type 2 diabetes. Alternative splicing results in multiple transcript variants. This gene was previously referred to as ANGPTL2 but has been renamed ANGPTL4.

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et al,Ischemic Stroke Risk Associated with Mitochondrial Haplogroup F in the Asian Population. In Cells on 2020 Aug 11 by Meng-Han Tsai, Chung-Wen Kuo, et al..PMID: 32796743, , (2020),
PMID: 32796743
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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