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位置: 首页 > 抗体 > P300/CBP Antibody

P300/CBP Antibody#33460

P300/CBP Antibody
P300/CBP Antibody
是否有货: 有货
产品总价:
产品详情

产品名称P300/CBP Antibody

来源种属Rabbit

克隆性Polyclonal

纯化The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

应用WB IHC

种属反应性Hu Ms Rt

特异性The antibody detects endogenous levels of total P300/CBP protein.

免疫原类型Peptide

免疫原描述Synthesized peptide derived from human P300/CBP.

基因/蛋白名称P300/CBP

别名CREB-binding protein; CREBBP E1A-associated protein p300; EP300; P300 transcription coactivator homolog;

数据库入口号Swiss-Prot: Q09472/Q92793
NCBI Gene ID: 2033

UniprotQ09472

实际分子量93kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C

应用详情

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from COLO205 cells, using P300/CBP antibody #33460.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using P300/CBP antibody #33460.
背景

Acetylates histones, giving a specific tag for transcriptional activation. Also acetylates non-histone proteins, like NCOA3 and FOXO1. Binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. Acts as a coactivator of ALX1 in the presence of EP300. Acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators: NPAS2-ARNTL/BMAL1 and CLOCK-ARNTL/BMAL1 heterodimers.

Gerd A. Blobel, J. Leukoc. Biol., Apr 2002; 71: 545.
Eric Kalkhoven, Hum. Mol. Genet., Feb 2003; 12: 441 - 450.
Wei-fang Shen, Mol. Cell. Biol., Nov 2001; 21: 7509 - 7522.
Frederick C. Nucifora, Science, Mar 2001; 291: 2423.

如果您使用该产品33460发表了文章,请通知我们,让我们可以引用您的文献。

et al,Cytoplasmic expression of p33ING1b is correlated with tumorigenesis and progression of head and neck squamous cell carcinoma. In Histol Histopathol on 2011 May by Xiao-Han Li, Akira Noguchi, et al..PMID: 21432775, , (2011),
PMID: 21432775
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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