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SPI1 Antibody#33699

SPI1 Antibody
是否有货: 有货
产品总价:
产品详情

产品名称SPI1 Antibody

来源种属Rabbit

克隆性Polyclonal

纯化The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

应用WB

种属反应性Hu Ms Rt

特异性The antibody detects endogenous levels of total SPI1 protein.

免疫原类型Peptide

免疫原描述Synthesized peptide derived from internal of human SPI1.

基因/蛋白名称SPI1

别名31 kDa transforming protein; SPI1; transcription factor PU.1;

数据库入口号Swiss-Prot: P17947
NCBI Gene ID: 6688

UniprotP17947

实际分子量42kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C

应用详情

Western blotting: 1:500~1:3000

Western blot analysis of extracts from HepG2 and 293 cells, using SPI1 antibody #33699.
背景

Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing By similarity.

Ray D., Oncogene 5:663-668(1990).
Rao S., J. Biol. Chem. 274:11115-11124(1999).
Mao S., Mol. Cell. Biol. 19:3635-3644(1999).

如果您使用该产品33699发表了文章,请通知我们,让我们可以引用您的文献。

et al,Knockdown of PU.1 AS lncRNA Inhibits Adipogenesis Through Enhancing PU.1 mRNA Translation.In J Cell Biochem on 2013 Nov by Wei-Jun Pang, Li-Gen Lin,et al..PMID:23749759, , (2013),
PMID: 23749759
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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