产品详情
产品名称STAT3(Phospho-Ser727) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB IHC IF
种属反应性Hu Ms Rt
特异性The antibody detects endogenous level of STAT3 only when phosphorylated at serine 727.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of serine 727 (P-M-S(p)-P-R) derived from Human STAT3.
基因/蛋白名称STAT3
修饰Phospho
别名APRF, HIES
数据库入口号Swiss-Prot: P40763
NCBI Protein: NP_003141.2
UniprotP40763
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 88kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from Hela and HepG2 cells untreated or treated with PMA usingSTAT3(Phospho-Ser727) Antibody #11046.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT3(Phospho-Ser727) Antibody #11046(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using STAT3(Phospho-Ser727) Antibody #11046.
Western blot analysis of extracts from Hela cells, treated with IFNa or calf intestinal phosphatase (CIP), using STAT3 (Phospho-Ser727) Antibody #11046.
背景
Transcription factor that binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA.
Heim M H, (1999) J Recept Signal Transduct Res. 19: 75-120.
Takeda K, et al. (1997) Proc Natl Acad Sci USA. 94: 3801-3804.
Catlett-Falcone R, et al. (1999) Immunity. 10: 105-115.
Garcia R, et al. (1998) J Biomed Sci. 5: 79-85.
Bromberg J F, et al. (1999) Cell. 98: 295-303.
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