产品详情
产品名称ASC Polyclonal Antibody
来源种属Rabbit
克隆性Polyclonal
纯化The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
应用WB IHC IF ELISA
种属反应性Hu Ms Rt
特异性ASC Polyclonal Antibody detects endogenous levels of ASC protein.
免疫原描述Synthesized peptide derived from the N-terminal region of human ASC.
基因/蛋白名称ASC
别名PYCARD; ASC; CARD5; TMS1; Apoptosis-associated speck-like protein containing a CARD; hASC; Caspase recruitment domain-containing protein 5; PYD and CARD domain-containing protein; Target of methylation-induced silencing 1
数据库入口号Swiss-Prot: Q9ULZ3
NCBI Gene ID: 29108
UniprotQ9ULZ3
实际分子量21kd
浓度1mg/ml
配方Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
保存Store at -20˚C/1 year
应用详情
Western Blot: 1/500 - 1/2000.
Immunohistochemistry: 1/100 - 1/300.
Immunofluorescence: 1/200 - 1/1000.
ELISA: 1/40000.
Not yet tested in other applications.
Western Blot analysis of 823 293T cells using ASC Polyclonal Antibody
Wu, Dong-Dong, et al. "Inhibition of alveolar macrophage pyroptosis reduces lipopolysaccharide-induced acute lung injury in mice." Chinese medical journal 128.19 (2015): 2638.
Western blot analysis of lysates from 1) K562 , 2) Hela , 3) 3T3 cells, （Green） primary antibody was diluted at 1:1000, 4°over night, secondary antibody was diluted at 1:10000, 37° 1hour. （Red） Tubulin β Monoclonal Antibody(5G3) antibody was diluted at 1:5000 as loading control, 4° over night,secondary antibody was diluted at 1:10000, 37° 1hour.
Western Blot analysis of various cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour). Cell lysate was extracted by Minute™ Plasma Membrane Protein Isolation and Cell Fractionation Kit(SM-005, Inventbiotech,MN,USA).
Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,ASC Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1,ASC Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using ASC Antibody. The picture on the right is blocked with the synthesized peptide.
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et al,The Extracts of Angelica sinensis and Cinnamomum cassia from Oriental Medicinal Foods Regulate Inflammatory and Autophagic Pathways against Neural Injury after Ischemic Stroke. In Oxid Med Cell Longev on 2021 Jun 26 by Cheng Luo, Qi Chen,et al..PMID:34257822,
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et al,Ginsenoside Rk3 alleviated DSS-induced ulcerative colitis by protecting colon barrier and inhibiting NLRP3 inflammasome pathway. In Int Immunopharmacol on 2020 Aug by Mi Tian, Pei Ma, et al..PMID: 32521491,
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