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位置: 首页 > Akt信号通路 > Akt(Ab-308) Antibody

Akt(Ab-308) Antibody#21055

Akt(Ab-308) Antibody
Akt(Ab-308) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称Akt(Ab-308) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

应用WB IHC

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of total Akt protein.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around aa.306~310 (M-K-T-F-C) derived from Human AKT1.

基因/蛋白名称Akt

别名 RAC-PK-alpha; Protein kinase B;

数据库入口号Swiss-Prot: P31749
NCBI Protein: NP_001014431.1

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 60kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from HepG2 and Hela cells using Akt(Ab-308) Antibody #21055.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Akt(Ab-308) Antibody #21055(left) or the same antibody preincubated with blocking peptide(right).
背景

General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.

Tremblay F, et al. (2005) Diabetes; 54(9): 2674-84.
Xu BE, et al. (2005) J Biol Chem; 280(40): 34218-23.
Samuels Y, et al. (2005) Cancer Cell; 7(6): 561-73.
Di Maira G, et al. (2005) Cell Death Differ; 12(6): 668-77.

如果您使用该产品21055发表了文章,请通知我们,让我们可以引用您的文献。

et al,In Vitro and In Vivo Evaluation of Antidiabetic Properties and Mechanisms of Ficus tikoua Bur.. In Nutrients on 2022 Oct 20 by Hanlei Wang, Kun Zhang,et al..PMID:36297098, , (2022),
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PMID: 31994797
et al,Combination of Ruthenium Complex and Doxorubicin Synergistically Inhibits Cancer Cell Growth by Down-Regulating PI3K/AKT Signaling Pathway.In Front Oncol on 2020 Feb 18 by Lin K, Rong Y, et al..PMID:32133289, , (2020),
PMID: 32133289
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et al,Choline Prevents Fetal Overgrowth and Normalizes Placental Fatty Acid and Glucose Metabolism in a Mouse Model of Maternal Obesity.In J Nutr Biochem on 2017 Nov by Juha Nam , Esther Greenwald,et al..PMID: 28915389, , (2017),
PMID: 28915389
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查看更多
et al,Fatty Acid Synthase Regulates Proliferation and Migration of Colorectal Cancer Cells via HER2-PI3K/Akt Signaling Pathway.In Nutr Cancer on 2012 Aug by Nan Li, Xiaodong Bu,et al..PMID:22860766, , (2012),
PMID: 22860766
Massimo Nabissi, Maria Beatrice Morelli1, Consuelo Amantini et al,TRPV2 channel negatively controls glioma cell proliferation and resistance to Fas-induced apoptosis in ERK-dependent manner., Carcinogenesis, 31(5):794-803 (2010),
PMID: 20093382
et al,TRPV2 channel negatively controls glioma cell proliferation and resistance to Fas-induced apoptosis in ERK-dependent manner.In Carcinogenesis on 2010 May by Nabissi M, Morelli MB,et al..PMID:20093382, , (2010),
PMID: 20093382
et al,Suppression of Hypoxia-Induced HIF-1alpha Accumulation by VEGFR Inhibitors: Different Profiles of AAL993 Versus SU5416 and KRN633.In Cancer Lett on 2010 Oct 1 by Hyun Seung Ban, Masaharu Uno,et al..PMID:20378243, , (2010),
PMID: 20378243
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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