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位置: 首页 > 磷酸化抗体 > NFkB-p65(Phospho-Ser536) Antibody

NFkB-p65(Phospho-Ser536) Antibody#11014

NFkB-p65(Phospho-Ser536) Antibody
NFkB-p65(Phospho-Ser536) Antibody
NFkB-p65(Phospho-Ser536) Antibody
NFkB-p65(Phospho-Ser536) Antibody
NFkB-p65(Phospho-Ser536) Antibody
NFkB-p65(Phospho-Ser536) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称NFkB-p65(Phospho-Ser536) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC IF

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of NF-kB p65 only when phosphorylated at serine 536.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 536 (F-S-S(p)-I-A) derived from Human NFkB-p65.

基因/蛋白名称NFkB-p65

修饰Phospho

别名NFKB3; RELA; TF65; Transcription factor p65; p65

数据库入口号Swiss-Prot: Q04206
NCBI Protein: NP_001138610.1

UniprotQ04206

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 65kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from HepG2 and HL60 cells untreated or treated with TNF using NFkB-p65(Phospho-Ser536) Antibody #11014.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFκB-p65 (Phospho-Ser536) Antibody #11014 (left) or the same antibody preincubated with blocking peptide #51014 (right).
Immunofluorescence staining of methanol-fixed Hela cells using NFkB-p65(Phospho-Ser536) Antibody #11014.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFκB-p65 (Phospho-Ser536) Antibody #11014.
Immunohistochemical analysis of paraffin-embedded human Lung carcinoma tissue using NFκB-p65 (Phospho-Ser536) Antibody #11014.
Immunofluorescence staining of methanol-fixed MEF cells using NFκB-p65 (Phospho-Ser536) Antibody #11014.
背景

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex.

Doyle S L, et al. (2005) J Biol Chem. 280(25): 23496-23501.
Anwar K N, et al. (2004) J Immunol. 173(11): 6965-6972.
Baeuerle P A, et al. (1994) Annu Rev Immunol. 12:141-179.
Baeuerle P A, et al. (1996) Cell 87:13-20.
Haskill S, et al. (1991) Cell 65:1281-1289.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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