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位置: 首页 > KO/KD抗体 > p53 Mouse Monoclonal Antibody

p53 Mouse Monoclonal Antibody#38007

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p53 Mouse Monoclonal Antibody
p53 Mouse Monoclonal Antibody
p53 Mouse Monoclonal Antibody
p53 Mouse Monoclonal Antibody
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产品总价:
产品详情

产品名称p53 Mouse Monoclonal Antibody

来源种属Mouse

克隆性Monoclonal

克隆性6C4

纯化The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

应用WB IHC

种属反应性Hu

特异性p53 Mouse monoclonal antibody detects endogenous p53 proteins.

免疫原描述Synthetic peptide of human p53

基因/蛋白名称p53

别名Tumor suppressor p53; Phosphoprotein p53; Antigen NY-CO-13

数据库入口号Swiss-Prot#:P04637

UniprotP04637

实际分子量53kd

浓度1.0mg/ml

配方PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

保存Store at -20˚C

应用详情

Western blotting: 1:2000
Immunohistochemistry: 1:200

Western blot analysis of 293T, using #38007 diluted at 1:1,000 (left lane) and 1:2,000 (right lane).
IHC staining of paraffin-embedded Human colon cancer tissue with p53 mouse mAb(6C4) diluted at 1:200.
Figure legend: Western blot showing p53 (protein code: Tp53) expression in wild-type (WT) and Tp53 shRNA knockdown rat PC-12 cells. Beta-actin (Actb) served as the loading control. Conclusion: Since this antibody detects endogenous p53 protein in WT but not in shRNA knockdown cell lysate, this antibody is highly specific.
Western blot analysis of lysates from 1) 293T, 2) HEK293, 3) A431 cells using p53 Mouse Monoclonal Antibody #38007 (Green) and Actin β Polyclonal Antibody as the loading control (Red).
背景

p53 plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro.

如果您使用该产品38007发表了文章,请通知我们,让我们可以引用您的文献。

et al,Resveratrol suppresses human cervical carcinoma cell proliferation and elevates apoptosis via the mitochondrial and p53 signaling pathways.In Oncol Lett.On 2018 Jun by Li L, Qiu RL et al..PMID:29928358, , (2018),
PMID: 29928358
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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