抗体特异性最被接受和信任的验证方法之一是基因敲除验证,为确保我们的抗体特异并提供可重复的结果,我们最近引入了敲除 (KO/KD) 验证。使用人KO/KD细胞系,通过Knock out/Knock Down的方式来进行,在 KO 细胞系中测试特异性抗体时,特异性抗体不会产生信号,但在野生型 (WT) 细胞系测试特异性抗体时,特异性抗体会产生特异性靶蛋白信号。 通过RNA干扰技术敲降靶标基因的表达(Knock Down,KD)通过基因水平的调控,来改变蛋白表达水平,进而判断抗体与靶标的结合是否特异。

KO/KD Antibody Products

p53 Mouse Monoclonal Antibody

Figure legend: Western blot showing p53 (protein code: Tp53) expression in wild-type (WT) and Tp53 shRNA knockdown rat PC-12 cells. Beta-actin (Actb) served as the loading control.Conclusion: Since this antibody detects endogenous p53 protein in WT but not in shRNA knockdown cell lysate, this antibody is highly specific.#38007

AKT1S1 antibody

Western blotting analysis using AKT1S1 antibody #38768.

Integrin beta-1 Antibody

Western blotting analysis using Integrin beta-1 Antibody #48627.

C1QBP Polyclonal Antibody

Western blotting analysis using C1QBP Polyclonal Antibody #40661.

Cullin 4a Antibody

Western blotting analysis using Cullin 4a Antibody #49742.

Flotillin 1 Antibody

Western blotting analysis using Flotillin 1 Antibody #49861.

Calreticulin Antibody

Western blotting analysis using Calreticulin Antibody #48841.