产品详情
产品名称GFAP Mouse Monoclonal Antibody
来源种属Mouse
克隆性Monoclonal
纯化Affinity purification using immunogen.
应用WB IHC IF
种属反应性Rt Ms
特异性The GFAP Mouse Monoclonal antibody detects endogenous GFAP proteins.
基因/蛋白名称GFAP
别名FLJ45472; GFAP; Glial fibrillary acidic protein
数据库入口号Swiss-Prot#:P14136
UniprotP14136
实际分子量45kd
浓度1.0mg/ml
配方Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20˚C
应用详情
WB: 1:2000~1:5000
IHC: 1:50-300
IF: 1:100-1:200
Immunofluorescence analysis of Rat-brain tissue, using #38014 diluted at 1:200.
Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,GFAP Monoclonal Antibody(5C8) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1,GFAP Monoclonal Antibody(5C8) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,GFAP Monoclonal Antibody(5C8) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of Mouse-brain tissue,using #38014 diluted at 1:200.
Immunofluorescence analysis of Mouse-brain tissue. 1,GFAP Monoclonal Antibody(5C8)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of Rat-brain tissue. 1,GFAP Monoclonal Antibody(5C8)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Rat kidney tissue,using #38014 diluted at 1:200.
Western blot analysis of Rat Brain Tissue, using #38014 diluted at 1:5,000.
背景
GFAP is a member of the class III intermediate filament protein family. It is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes.
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