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位置: 首页 > 磷酸化抗体 > G3BP-1(Phospho-Ser232) Antibody

G3BP-1(Phospho-Ser232) Antibody#11082

G3BP-1(Phospho-Ser232) Antibody
G3BP-1(Phospho-Ser232) Antibody
G3BP-1(Phospho-Ser232) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称G3BP-1(Phospho-Ser232) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC IF

种属反应性Hu

特异性The antibody detects endogenous level of G3BP-1 only when phosphorylated at serine 232.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 232 (S-S-S(p)-P-A) derived from Human G3BP-1.

基因/蛋白名称G3BP-1

修饰Phospho

别名G3BP; GAP SH3-domain binding protein 1;

数据库入口号Swiss-Prot: Q13283
NCBI Protein: NP_005745.1

UniprotQ13283

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 60kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from 293 cells untreated or treated with starvation using G3BP-1(Phospho-Ser232) Antibody #11082.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using G3BP-1(Phospho-Ser232) Antibody #11082(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using G3BP-1(Phospho-Ser232) Antibody #11082.
背景
May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.

Tao WA, et al. (2005) Nat Methods. 2(8): 591-598.
Zheng H, et al. (2005) Mol Cell Proteomics. 4(6):721-730.
Beausoleil SA, et al. (2004) Proc Natl Acad Sci U S A.101 (33): 12130-12135.
Tourrihre H, et al. (2003) J Cell Biol.160 (6): 823-831.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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