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位置: 首页 > 磷酸化抗体 > Tau (Phospho-Ser516/199) Antibody

Tau (Phospho-Ser516/199) Antibody#12123

Tau (Phospho-Ser516/199) Antibody
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产品总价:
产品详情

产品名称Tau (Phospho-Ser516/199) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms Rt

特异性The antibody detects endogenous levels of Tau only when phosphorylated at serine 516/199.

免疫原类型peptide

免疫原描述Peptide sequence around phosphorylation site of serine 516/199 (Y-S-S(p)-P-G) derived from Human Tau.

基因/蛋白名称Tau

修饰Phospho

别名MAPT; Microtubule-associated protein tau; MTBT1; Neurofibrillary tangle protein; Paired helical filament-tau; PHF-tau

数据库入口号Swiss-Prot#:P10636;
NCBI Gene#:4137

UniprotP10636

实际分子量74kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C

应用详情
Western blotting: 1:500~1:3000
Western blot analysis of extracts from Mouse brain cells, using Tau (Phospho-Ser516/199) antibody #12123. The lane on the right is treated with the synthesized peptide.
背景
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

如果您使用该产品12123发表了文章,请通知我们,让我们可以引用您的文献。

et al,Capsaicin consumption reduces brain amyloid-beta generation and attenuates Alzheimer's disease-type pathology and cognitive deficits in APP/PS1 mice. In Transl Psychiatry on 2020 Jul 13 by Jun Wang, Bin-Lu Sun,et al..PMID:32661266, , (2020),
PMID: 32661266
et al,NeurotrophIn receptor p75 mediates amyloid β-Induced tau pathology. In Neurobiol Dis on 2019 Dec by Shen LL, Li WW, et al..PMID:31394202, , (2019),
PMID: 31394202
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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