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位置: 首页 > 磷酸化抗体 > ICAM-1(Phospho-Tyr512) Antibody

ICAM-1(Phospho-Tyr512) Antibody#11083

ICAM-1(Phospho-Tyr512) Antibody
ICAM-1(Phospho-Tyr512) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称ICAM-1(Phospho-Tyr512) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu

特异性The antibody detects endogenous level of ICAM-1 only when phosphorylated at tyrosine 512.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 512 (K-K-Y(p)-R-L) derived from Human ICAM-1.

基因/蛋白名称ICAM-1

修饰Phospho

别名ICA1; ICAM1;

数据库入口号Swiss-Prot: P05362
NCBI Protein: NP_000192.2

UniprotP05362

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 89 92kd
Western blotting: 1:500~1:1000
 

Western blot analysis of extracts from HUVEC cells using ICAM-1(Phospho-Tyr512) Antibody #11083(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).
Western blot analysis of extracts from C2C12 cells, treated with starve or calf intestinal phosphatase (CIP), using ICAM-1 (Phospho-Tyr512) Antibody #11083.
背景

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin a-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.

Greenwood J, et al. (2003) J Immunol; 171(4):2099-2108.
Zhou Z, et al. (2005) Eur J Pharmacol; 513(1-2):1-8.
Chen YH, et al. (2001) J Cell Biochem; 82(3):512-521
 

如果您使用该产品11083发表了文章,请通知我们,让我们可以引用您的文献。

et al,Impaired CD200鈥揅D200R-mediated microglia silencing enhances midbrain dopaminergic neurodegeneration: Roles of aging, superoxide, NADPH oxidase, and p38 MAPK. In Free Radic Biol Med on 2011 May 1 by Xi-Jin Wang, Shi Zhang, et al..PMID: 21295135, , (2011),
PMID: 21295135
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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