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位置: 首页 > 抗体 > c-Src Polyclonal Antibody

c-Src Polyclonal Antibody#40790

c-Src Polyclonal Antibody
c-Src Polyclonal Antibody
c-Src Polyclonal Antibody
c-Src Polyclonal Antibody
c-Src Polyclonal Antibody
c-Src Polyclonal Antibody
是否有货: 有货
产品总价:
产品详情

产品名称c-Src Polyclonal Antibody

来源种属Rabbit

克隆性Polyclonal

纯化The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

应用WB IHC IF ELISA

种属反应性Hu Ms Rt

特异性c-Src Polyclonal Antibody detects endogenous levels of c-Src protein.

免疫原描述Synthesized peptide derived from human c-Src around the non-phosphorylation site of Y419.

基因/蛋白名称c-Src

别名SRC; SRC1; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src; p60-Src

数据库入口号Swiss-Prot: P12931
NCBI Gene ID: 6714

UniprotP12931

实际分子量60kd

浓度1mg/ml

配方Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

保存Store at -20˚C/1 year

应用详情

Western Blot: 1/500 - 1/2000.
Immunohistochemistry: 1/100 - 1/300.
Immunofluorescence: 1/200 - 1/1000.
ELISA: 1/40000.
Not yet tested in other applications.

Western Blot analysis of 293 cells using c-Src Polyclonal Antibody
Western Blot analysis of various cells using c-Src Polyclonal Antibody
Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1,c-Src Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,c-Src Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of rat-lung tissue. 1,c-Src Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of rat-lung tissue. 1,c-Src Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

如果您使用该产品40790发表了文章,请通知我们,让我们可以引用您的文献。

et al,YAP1 plays a key role of the conversion of normal fibroblasts into cancer-associated fibroblasts that contribute to prostate cancer progression.In J Exp Clin Cancer Res on 2020 Feb 17; by Shen T, Li Y et al..PMID:32066485, , (2020),
PMID: 32066485
et al,A thiazole-derived oridonIn analogue exhibits antitumor activity by directly and allosterically InhibitIng STAT3. In J Biol Chem on 2019 Nov 15;by Shen X, Zhao L,et al..PMID:31594861, , (2019),
PMID: 31594861
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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