产品详情
产品名称JNK1 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Immunogen affinity purified
应用WB, ICC, IHC, FC
种属反应性Hu,Ms,Rt, zebrafish
免疫原描述peptide
别名AI849689 antibody
c Jun N terminal kinase 1 antibody
C-JUN kinase 1 antibody
c-Jun N-terminal kinase 1 antibody
EC 2.7.11.24 antibody
JNK 1 antibody
JNK antibody
JNK-46 antibody
JNK1A2 antibody
JNK21B1/2 antibody
MAP kinase 8 antibody
MAPK 8 antibody
mapk8 antibody
Mitogen activated protein kinase 8 antibody
Mitogen-activated protein kinase 8 antibody
MK08_HUMAN antibody
p54 gamma antibody
Prkm8 antibody
Protein kinase JNK1 antibody
Protein kinase, mitogen-activated, 8 antibody
SAPK 1 antibody
SAPK gamma antibody
SAPK1 antibody
Stress-activated protein kinase 1 antibody
Stress-activated protein kinase JNK1 antibody
数据库入口号Swiss-Prot#:P45983
计算分子量~48/54kDa
配方1*TBS (pH7.4), 0.5%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC: 1:50-1:100
Western blot analysis of JNK1 on different cell lysates using anti-JNK1 antibody at 1/1,000 dilution. Positive control: Lane 1: K562 Lane 2: PC-12 Lane 3: Hela
Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-BDNF antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-BDNF antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-BDNF antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-BDNF antibody. Counter stained with hematoxylin.
ICC staining of BNDF in A172 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining of BNDF in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining of BNDF in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
背景
Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity.
背景文献
1."JNK1-mediated phosphorylation of Bcl-2 regulates starvation-induced autophagy." Wei Y., Pattingre S., Sinha S., Bassik M., Levine B. Mol. Cell 30:678-688(2008)
2."JNK phosphorylates Yes-associated protein (YAP) to regulate apoptosis." Tomlinson V., Gudmundsdottir K., Luong P., Leung K.Y., Knebel A., Basu S. Cell Death Dis. 1:E29-E29(2010)
Yes
