产品详情
产品名称xanthine dehydrogenase Antibody
来源种属Mouse
克隆性Monoclonal
克隆性1C8
纯化ProG affinity purified
应用IHC,FC
种属反应性Hu
免疫原描述Peptide
别名Xanthine dehydrogenase antibody
Xanthine dehydrogenase/oxidase antibody
Xanthine oxidase antibody
Xanthine oxidoreductase antibody
XD antibody
XDH antibody
XDH_HUMAN antibody
xdha antibody
XO antibody
xor antibody
数据库入口号Swiss-Prot#:P47989
计算分子量146 kDa
配方1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
IHC: 1:50-1:200
FC: 1:50-1:100
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-xanthine dehydrogenase antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-xanthine dehydrogenase antibody. Counter stained with hematoxylin.
Flow cytometric analysis of LOVO cells with xanthine dehydrogenase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the secondary antibody.
背景
The process of metabolizing purines to a common molecule known as xanthine is an essential process for the proper shuttling of uric acid. Xanthine oxidase is a flavoprotein enzyme that coordinates molybdenum and utilizes NAD+ as an electron acceptor to catalyze the oxidation of hypoxanthine to xanthine and then to uric acid. The predominant form of this enzyme is xanthine dehydrogenase, which is a homodimer that can be converted to xanthine oxidase by sulfhydryl oxidation or proteolytic modification. Xanthine oxidase is present in species ranging from bacteria to human and is ubiquitously expressed in mammalian tissues. In the oxidase form, this enzyme is coupled to the generation of free radicals. Individuals showing marked elevation of serum xanthine oxidase is suggestive of chronic liver disease and cholestasis, which is a condition defined by hepatic obstruction. Hepatic obstruction causes bile salts, the bile pigment bilirubin, and fats to accumulate in the blood stream instead of being eliminated normally. The clinical consequences of defects in xanthine oxidase range from mild to severe and even contribute to fatal disorders.
背景文献
1. Brand S et al. Oxidative DNA damage in kidneys and heart of hypertensive mice is prevented by blocking angiotensin II and aldosterone receptors. PLoS One 9:e115715 (2014).
2. Brem R et al. Oxidation-mediated DNA cross-linking contributes to the toxicity of 6-thioguanine in human cells. Cancer Res 72:4787-95 (2012).